Plasmids play an important role in the virulence and antibiotic resistance of Salmonella. Salmonella carry plasmids having different size and number. Plasmid profile analysis of single isolates of Salmonella typhimurium and Salmonella enteritidis revealed the presence of 4- plasmid profiles. Salmonella serovars produce several Type III secretions including Sop-E and Sop –B. Soap E is detected b...

Immunodominant antigens of an approximate molecular mass of 27 kDa (FG 27) were obtained from an excretory-secretory product of adult Fasciola gigantica by a simple continuous-elution method. A dot-ELISA using the FG 27 antigen was developed for the detection of specific antibodies from patients infected with F. gigantica. Control sera were obtained from patients with other parasitic infections...

Leptospirosis is worldwide zoonotic disease that is serious problem in south Indian states and now emerging in North India. The focus these days is to develop recombinant outer membrane protein based Enzyme-linked immunosorbent assay (ELISA) for seroprevalence of leptospirosis. The aim of this study was to evaluate indirect ELISA based on recombinant LipL41, Loa22, LipL21, LipL32 and OmpL1 prot...

Outbreaks of the Southern rice black-streaked dwarf virus (SRBSDV) have caused significant losses in many rice-growing areas Vietnam, especially both North and Central Vietnam recent years. To detect virus, traditional reverse transcription polymerase chain reaction (RT-PCR) methodology immunoassays are currently employed. RT-PCR is accurate but requires expensive chemicals instruments, as well...

Outbreaks of the southern rice black-streaked dwarf virus (SRBSDV) have caused significant crop losses in southern China in recent years, especially in 2010. There are no effective, quick and practicable methods for the diagnosis of rice dwarf disease that can be used in the field. Traditional reverse transcription-polymerase chain reaction (RT-PCR) methodology is accurate but requires expensiv...

Canine distemper is the most prevalent viral disease of dogs, which has high mortality rate. Vaccination of dogs against canine distemper is the only measure to control the disease. The aim of the present study was to find out the effect of commercial live attenuated vaccines in the control of distemper and the importance of the annual vaccination against distemper based on the occurrence of in...

Human epidermal growth factor receptor 2 (HER2) is one of the most important prognostic and predictive factors for breast cancer patients. Recently, serum HER2 ECD level of patients detected by enzyme-linked immunoabsorbent assay (ELISA) has been shown to predict tumor HER2 status and reveal its association with tumor progression, recurrence and poor prognosis. In this study, we established a n...

Background: A test strip IgM Dot-ELISA assay for the detection of leptospirespecific IgM antibodies in human sera was developed. Antigen dotted on a nitrocellulose paper strip was the sonicated antigen prepared from Leptospira serovar prevalent in this area, i.e., Icterohaemmorhagiae. Objective: The aim of this study was to evaluate an inhouse prepared IgM DotELISA test strip for the diagnosis ...