" One-step RNA pathogen detection with reverse transcriptase activity of a mutated thermostable Thermus aquaticus DNA polymerase. " EMBO through an abasic DNA lesion: structural basis for adenine selectivity. " A. " Mutant DNA polymerase for improved detection of single-nucleotide variations in microarrayed primer extension " Chem. Eur. A. " Increased single-nucleotide discrimination in allele-...

The one-electron oxidation of duplex DNA generates a nucleobase radical cation (electron "hole") that migrates long distances by a hopping mechanism. The radical cation reacts irreversibly with H2O or O2 to form oxidation products (damaged bases). In normal DNA (containing the four common DNA bases), reaction occurs most frequently at guanine. However, in DNA duplexes that do not contain guanin...

Molecular tips in scanning tunneling microscopy can directly detect intermolecular electron tunneling between sample and tip molecules and reveal the tunneling facilitation through chemical interactions that provide overlap of respective electronic wave functions, that is, hydrogen-bond, metal-coordination-bond, and charge-transfer interactions. Nucleobase molecular tips were prepared by chemic...

A chelator-type beta-C-nucleoside having a catechol ligand as the nucleobase was found to form a stable 2:1 complex with trimethyl borate, which was characterized by 1H-NMR and electrospray ionization time-of-flight (ESI-TOF) mass spectroscopies. Both phosphoramidite and phosphotriester derivatives of this nucleoside were also prepared as synthetic precursors for DNA oligomer syntheses.

Thiols have a strong tendency to be chemisorbed to gold surfaces forming self-assembled monolayers. 2] The feature of strong S Au bond formation has been exploited in numerous applications including the immobilisation of DNA on gold surfaces for the generation of DNA microarrays or gold electrodes for measuring charge migration mediated by double stranded DNA. To be immobilised on gold, DNA has...