نتایج جستجو برای: dna modification

تعداد نتایج: 624139  

2010
Tiegang Xu Fen Yao Xiufen Zhou Zixin Deng Delin You

A novel, site-specific, DNA backbone S-modification (phosphorothioation) has been discovered, but its in vivo function(s) have remained obscure. Here, we report that the enteropathogenic Salmonella enterica serovar Cerro 87, which possesses S-modified DNA, restricts DNA isolated from Escherichia coli, while protecting its own DNA by site-specific phosphorothioation. A cloned 15-kb gene cluster ...

Journal: :The Journal of clinical investigation 2003
Dieter C Gruenert Emanuela Bruscia Giuseppe Novelli Alessia Colosimo Bruno Dallapiccola Federica Sangiuolo Kaarin K Goncz

Small DNA fragments have been used to modify endogenous genomic DNA in both human and mouse cells. This strategy for sequence-specific modification or genomic editing, known as small-fragment homologous replacement (SFHR), has yet to be characterized in terms of its underlying mechanisms. Genotypic and phenotypic analyses following SFHR have shown specific modification of disease-causing geneti...

Journal: :Nucleic acids research 1998
A Boybek T D Ray M C Evans P J Dyson

Both Streptomyces lividans and Streptomyces avermitilis encode similar systems of post-replicative DNA modification which act site-specifically on closely opposed guanines on either strand. The modifications can be detected since they react in vitro with an oxidative derivative of Tris, resulting in strand cleavage. Previous analysis of the preferred modification site of plasmid pIJ101 indicate...

2016
Wenxian Lan Zhongpei Hu Jie Shen Chunxi Wang Feng Jiang Huili Liu Dewu Long Maili Liu Chunyang Cao

DNA phosphorothioate (PT) modification, with sulfur replacing a nonbridging phosphate oxygen in a sequence and stereo specific manner, is a novel physiological variation in bacteria. But what effects on DNA properties PT modification has is still unclear. To address this, we prepared three double-stranded (ds) DNA decamers, d(CG(PX)GCCGCCGA) with its complementary strand d(TCGGCG(PX)GCCG) (wher...

Journal: :Neuroscience letters 2004
Jennifer Drake Robin Petroze Alessandra Castegna Qunxing Ding Jeffrey N Keller William R Markesbery Mark A Lovell D Allan Butterfield

There is increasing evidence of DNA oxidation and altered DNA repair mechanisms in Alzheimer's disease (AD) brain. Histones, which interact with DNA, conceivably could provide a protective shield for DNA against oxidative stress. However, because of their abundant lysine residues, histones may be a target for 4-hydroxynonenal (HNE) modification. In this study, we have shown that HNE binds to hi...

Journal: :Chemical communications 2012
Ganesh N Nawale Kiran R Gore Claudia Höbartner P I Pradeepkumar

The dual modified nucleotide 4'-C-aminomethyl-2'-O-methylthymidine 5'-triphosphate was synthesized and enzymatically incorporated into DNA by the thermophilic DNA polymerases Pfu and Therminator III. The dual ribose modification imparted increased exonuclease resistance to DNA compared to the well-known 2'-O-methyl modification.

Epigenetic modification is one of the effective factors in tumorigenesis. Epigenetic processes, especially aberrant DNA methylation, play important role in thyroid cancer, and many tumor suppressor genes including PTEN, RASSF1A and TIMP3 are aberrantly methylated and silenced in thyroid cancer. Because of the specified pattern of DNA methylation in various tumor cells, it is suggested that thes...

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