This study investigated the genetic diversity of morchella species using randomly amplified polymorphic DNA markers. Morchella species are an important group of edible mushrooms belonging to the family Helveliaceae with medicinal and economical significance. In this study we have developed an efficient method of genomic DNA isolation which was amplified by eight RAPD primers to test the polymor...

DNA isolation from some fungal organisms is difficult because they have cell walls or capsules that are relatively unsusceptible to lysis. Beginning with a yeast Saccharomyces cerevisiae genomic DNA isolation method, we developed a 30-min DNA isolation protocol for filamentous fungi by combining cell wall digestion with cell disruption by glass beads. High-quality DNA was isolated with good yie...

Fleischmann, A. & Heubl, G. 2009. Overcoming DNA extraction problems from carnivorous plants. Anales Jard. Bot. Madrid 66(2): 209-215. We tested previously published protocols for DNA isolation from plants with high contents of polyphenols and polysaccharides for several taxa of carnivorous plants. However, we did not get satisfying results with fresh or silica dried leaf tissue obtained from f...

PCR-based methods for the analysis of genomic DNA are becoming increasingly common both in research and for routine purposes. A rapid, small-scale DNA isolation method is needed to take full advantage of the speed and automation potential of the PCR technology. We demonstrate the use of Dynabeads DNA DIRECT, a kit for the isolation of PCR-ready genomic DNA from whole blood, bone marrow or cultu...

Here we describe a method for the isolation of PCR-ready genomic DNA from various zebrafish tissues that is based on a previously published murine protocol. The DNA solutions are of sufficient quality to allow PCR detection of transgenes from all commonly used zebrafish tissues. In sperm, transgene amplification was successful even when diluted 1000-fold, allowing easy identification of transge...

Isolation of nucleic acids from Chlorella is difficult, given the chemically complex nature of their cell walls and variable production of metabolites. Immobilization of microalgae in polymers adds additional difficulty. Here, we modified, amended, and standardized methods for isolation of nucleic acids and compared the yield of DNA and RNA from free-living and encapsulated microalgae C. soroki...

An efficient DNA isolation protocol specifically modified to get pure quality DNA required for molecular studieshas been reported in this paper. Some aquatic plants (Potamogeton spp., Ceratophyllum demersum and Myriophyllum spicatum) were used for the study. The protocol developed will be useful in getting high and pure DNA. Instead of using the available DNA extraction kits, this protocol can ...

We report here the isolation and complete genome sequencing of a large double-stranded DNA virus, Powai Lake megavirus, for the first time from India. The isolation of a large DNA virus with genome size >1 Mb from India further attests to the prevalence of Giant viruses in different environmental niches.

BACKGROUND Fragments of DNA from cells dying throughout the body are detectable in urine (transrenal DNA, or Tr-DNA). Our goal was the optimization of Tr-DNA isolation and detection techniques, using as a model the analysis of fetal DNA in maternal urine. METHODS We isolated urinary DNA using a traditional silica-based method and using a new technique based on adsorption of cell-free nucleic ...