A two-step purification of pneumococcal eclipse complex is described, which uses sucrose gradient sedimentation followed by agarose gel permeation chromatography. Purified complex contains, in addition to donor DNA single strands, macromolecular material that can be labeled with methionine or leucine during development of competence. This material co-chromatographed with eclipse complex DNA on ...

Two DNA polymerases have been purified from the 105,000 x g supernatant of ungerminated wheat. The purification stages included: high speed centrifugation, salt fractionation, DEAE-cellulose chromatography, Sephadex G-150 filtration and phosphocellulose chromatography. Several properties of the two enzyme (called A and B according to the order of elution from the phosphocellulose column) have b...

A hyper-osmotic electrotransformation method was developed for strain Bacillus subtilis. Sorbitol and mannitol are included in the electroporation medium recovery medium. In this study, optimised to increase transformation efficiency of B. subtilis 5434 (non-transformable by chemical methods) 430 fold, with a maximum value 8.6 ⋅ 105 CFU/µg integrative plasmid DNA. With setted 25 µF, 23 kV/cm, 2...

A number of criteria were used--chromatography on columns with single-stranded and double-stranded DNA, electrophoresis, peptide analysis, immunological tests and thermal denaturation of DNA--to show that protein (high mobility group) HMG1 and an unwinding protein from calf thymus are two distinct, unrelated proteins. While both proteins are thought to be related to DNA replication this might i...

The Escherichia coli AlkB protein is a 2-oxoglutarate/Fe(II)-dependent demethylase that repairs alkylated single stranded and double stranded DNA. Immunoaffinity chromatography coupled with mass spectrometry identified RecA, a key factor in homologous recombination, as an AlkB-associated protein. The interaction between AlkB and RecA was validated by yeast two-hybrid assay; size-exclusion chrom...

electrophoresis for detection of DNA point mutations. Journal of Chromatography B 697: 195}205. Righetti PG and GelR C (1998) Analysis of clinicallyrelevant, diagnostic DNA by capillary zone and double-gradient gel slab electrophoresis. Journal of Chromatography A 806: 97}112. Thormann W (1997) Drug monitoring by capillary electrophoresis. In Wong SHY, Sunshine I (Eds) Analytical Therapeutic Dr...

A group of DNA-binding proteins from the soluble extract of newborn rat epidermis have been separated by chromatography using DNA-cellulose columns. The electrophoretogram of the DNA-binding proteins eluted from a single stranded DNA-cellulose column shows five major proteins of molecular weights ranging between 25K to 40K. Both the epidermal protein filaggrin and most keratins, except two high...

Many biochemical, biomedical, and material applications hinge on the ability to effectively separate purify nanoscale materials. Though this need is largely addressed with biological macromolecules using a variety of chromatographic electrophoretic purification techniques, such techniques are usually laborious, time-consuming, often require complex costly instalments that inaccessible most labo...

An improved high-performance liquid chromatography/fluorometric assay has been established to quantitate the benzo(«)pyrene (BP) tetrols released after acid hydrolysis of lung DN A from lung cancer patients, so that the formation of benzo(a)pyrene diol-epoxide-DNA adducts can be measured. The r-7,c-10,f-8,f-9-tetrahydroxy-7,8,9,10-tetrahydro-BP isolated by high-performance liquid chromatograph...

research on ancient dna (adna) has the potential to enable molecular biologists and archeologists to decipher certain aspects of history by direct looking into the past. however, several major problems in this field limit the applicability of adna studies, most importantly contamination with modern dna and postmortem dna degradation. in this study we extracted and analyzed adna obtained from ~3...