Protein S-glutathionylation is a reversible redox modification that regulates mitochondrial metabolism and reactive oxygen species (ROS) production in liver and cardiac tissue. However, whether or not it controls ROS release from skeletal muscle mitochondria has not been explored. In the present study, we examined if chemically-induced protein S-glutathionylation could alter superoxide (O2●-)/h...

A valuable strategy to develop new therapeutic options for a variety of diseases has been the identification of new targets and applications for already approved drugs, the so-called drug repositioning. Recurrent ovarian cancer is a nearly incurable malignancy for which new and effective treatments are urgently needed. The alcohol-deterring drug disulfiram has been shown to cause preferential c...

Blocking aldehyde dehydrogenase with the drug disulfiram leads to an accumulation of intracellular acetaldehyde, which negatively affects the viability of the yeast Saccharomyces cerevisiae. Mutants of the yeast gene PSO2, which encodes a protein specific for repair of DNA interstrand cross-links, showed higher sensitivity to disulfiram compared to the wild type. This leads us to suggest that a...

The effects of disulfiram, its metabolite diethyldithiocarbamate and dithiodipyridine on alcohol metabolism of equine hepatic alcohol dehydrogenase (EC.1.1.1.1.) have been investigated. They were found to form enzyme-NAD(+)-inhibitor complexes which were competitive inhibitors of alcohol metabolism with dissociation constants (KEO,I) at pH 7.0 of 50 microM, 1.3 mM, and 260 microM, respectively....

Treatment of guinea-pigs with disulfiram for 8 days resulted in a significant enhancement of the in vitro activities of the N-demethylation of aminopyrine and the glucuronyl conjugation of p-mtrophenol. This increase was accompanied by an induction of liver growth and an increase in microsomal protein content. It might be concluded that disulfiram has inductive properties with respect to drug m...

Treatment of guinea-pigs with disulfiram for 8 days resulted in a significant enhancement of the in vitro activities of the N-demethylation of aminopyrine and the glucuronyl conjugation of p-mtrophenol. This increase was accompanied by an induction of liver growth and an increase in microsomal protein content. It might be concluded that disulfiram has inductive properties with respect to drug m...

Ingestion of ethanol in the presence of disulfiram may cause a histamine-like reaction due to accumulation of acetaldehyde. These disulfiram-ethanol reactions (DERs) are manifested by hypotension, tachycardia, gastritis, and angioedema. Fomepizole, an inhibitor of alcohol dehydrogenase, may halt progression of this reaction by blocking ethanol metabolism to acetaldehyde. We present 2 cases of d...

Sir: We describe the results of disulfiram im plantation over a five year period within clinical practice at an alcohol service at a district general hospital. The pharmacological basis of usage of disulfir am lies in its action on alcohol dehydrogenase preventing breakdown of acetaldehyde In the metabolism of ethanol. Early uncontrolled stud ies of disulfiram implantation showed significant im...

Treatment of guinea-pigs with disulfiram for 8 days resulted in a significant enhancement of the in vitro activities of the N-demethylation of aminopyrine and the glucuronyl conjugation of p-mtrophenol. This increase was accompanied by an induction of liver growth and an increase in microsomal protein content. It might be concluded that disulfiram has inductive properties with respect to drug m...

Treatment of guinea-pigs with disulfiram for 8 days resulted in a significant enhancement of the in vitro activities of the N-demethylation of aminopyrine and the glucuronyl conjugation of p-mtrophenol. This increase was accompanied by an induction of liver growth and an increase in microsomal protein content. It might be concluded that disulfiram has inductive properties with respect to drug m...