Focal adhesion (FA) disassembly required for optimal cell migration is mediated by microtubules (MTs); targeting of FAs by MTs coincides with their disassembly. Regrowth of MTs, induced by removal of the MT destabilizer nocodazole, activates the Rho-like GTPase Rac, concomitant with FA disassembly. Here, we show that the Rac guanine nucleotide exchange factor (GEF) Sif and Tiam1-like exchange f...

Abstract: In order to improve the product disassembly efficiency, the disassembly line balancing problem (DLBP) is transformed into a problem of searching for the optimum path in the directed and weighted graph by constructing the disassembly hierarchy information graph (DHIG). Then, combining the characteristic of the disassembly sequence, an entropy-based adaptive hybrid particle swarm optimi...

The primary cilium is a non-motile microtubule-based organelle that protrudes from the surface of most human cells and works as a cellular antenna to accept extracellular signals. Primary cilia assemble from the basal body during the resting stage (G0 phase) and simultaneously disassemble with cell cycle re-entry. Defective control of assembly or disassembly causes diverse human diseases includ...

Many proteins are known to promote ciliogenesis, but mechanisms that promote primary cilia disassembly before mitosis are largely unknown. Here we identify a mechanism that favours cilium disassembly and maintains the disassembled state. We show that co-localization of the S/G2 phase kinase, Nek2 and Kif24 triggers Kif24 phosphorylation, inhibiting cilia formation. We show that Kif24, a microtu...

Asymmetric disassembly of the synaptonemal complex (SC) is crucial for proper meiotic chromosome segregation. However, the signaling mechanisms that directly regulate this process are poorly understood. Here we show that the mammalian Rho GEF homolog, ECT-2, functions through the conserved RAS/ERK MAP kinase signaling pathway in the C. elegans germline to regulate the disassembly of SC proteins...

Non-motile primary cilium is an antenna-like structure whose defect is associated with a wide range of pathologies, including developmental disorders and cancer. Although mechanisms regulating cilia assembly have been extensively studied, how cilia disassembly is regulated remains poorly understood. Here, we report unexpected roles of Dishevelled 2 (Dvl2) and interphase polo-like kinase 1 (Plk1...

In Europe, EU regulation such as the European Waste Electrical and Electronic Equipment (WEEE) directive makes Original Equipment Manufacturers formally responsible for the set-up of collection and recovery systems. These stringent European directives on consumer waste will result in an increasing demand for cost efficient disassembly systems at a high capacity. The European WEEE directive does...

In this paper, we study disassembly sequencing flexibility that means the possibility of interchanging the order in which the required disassembly operations are performed. A mathematical model to design joint cellular disassembly systems is proposed, in which the trade-off of throughput time performances between the case of considering and the case of not considering disassembly sequencing fle...

Assembly/disassembly planning of mechanical products is one of the important manufacturing activities that must be supported by computers. Furthermore, recently, the design for disassemblability/recyclability becomes more important from the viewpoint of Life Cycle Assessment or Emission-Minimum. Therefore, we develop a system which can generate disassembly sequences and which can be applied to ...

Controlled assembly of nanomaterials has been the focus of much research. In contrast, controlled disassembly has not received much attention, even though both processes have been shown to be important in biology. By using a Pb2+-dependent RNA-cleaving DNAzyme, we demonstrate here control of the disassembly of gold nanoparticle aggregates in response to Pb2+. In the process, we show that nanopa...