High molecular weight glutenin subunits (HMW-GSs) play a major role in determining the dough quality of wheat. As D genome donor hexaploid wheat, Aegilops tauschii is an important genetic resource for wheat breeding. In present study, novel HMW-GSs from Ae. was identified and designated as Glu-Dt1. Multiple sequence alignment indicated that one cysteine mutated into arginine y-type subunit. Sit...

In replication-limited cells of Bacillus subtilis, Mfd is mutagenic at highly transcribed regions, even in the absence of bulky DNA lesions. However, the mechanism leading to increased mutagenesis through Mfd remains currently unknown. Here, we report that Mfd may promote mutagenesis in nutritionally stressed B. subtilis cells by coordinating error-prone repair events mediated by UvrA, MutY and...

Forward genetic analysis is one of the principal advantages of the zebrafish model system. However, managing zebrafish mutant lines derived from mutagenesis screens and mapping the corresponding mutations and integrating them into the larger collection of mutations remain arduous tasks. To simplify and focus these endeavors, we developed an approach that facilitates the rapid mapping of new zeb...

The RecA protein has a second, direct role in the mutagenesis of Escherichia coli and bacteriophage lambda in addition to its first, indirect role of inducing the SOS system by enhancing the proteolytic cleavage of the LexA repressor protein. The need for RecA protease and recombinase functions in the direct role was examined in cells containing split-phenotype RecA mutations, in the absence of...

introduction: active packaging is one of the innovations in food packaging industry that would be a respons to the changing needs of consumers and markets. in this technique, materials such as adsorbents oxygen, moisture, carbon dioxide, ethylene and carbon dioxide-releasing flavor compounds, antimicrobials, antioxidants and various aromatic compounds are used. in the last decade, great attenti...

clavulanic acid is produced industrially by fermentation of streptomyces clavuligerus and researches have increased its production by strain improvement, recombinant dna technology, and media composition and growth condition optimization. the main objective of this study was to increase the level of clavulanic acid production from streptomyces clavuligerus (dsm 738), using uv irradiation. after...

objective(s):[p1]  this study highlights xylanase overproduction from bacillus mojavensis via uv mutagenesis and optimization of the production process. materials and methods:bacillus mojavenis ptcc 1723 underwent uv radiation. mutants’ primary screening was based on the enhanced hollow zone diameter/ colony diameter ration (h/c ratios) of the colonies in comparison with the wild strain on xyla...