Purpose To assess whether a panel of serum pemphigoid autoantibody tests could be used to confirm an immunopathologic diagnosis mucous membrane (MMP) in direct immunofluorescent negative (DIF–) MMP patients. Design Prospective cross-sectional study. Participants Seventy-six patients with multisite 45 matched control participants. Methods Enzyme-linked immunosorbent assays (ELISAs) for BP180 and...

Chlamydiae are now commonly detected in genital tract specimens in routine diagnosis by direct immunofluorescence. Direct immunofluorescence is as sensitive and specific as conventional culture.' Studies in our laboratory also suggest that direct immunofluorescence may be a valuable test for cure.2 One of the problems encountered with direct immunofluorescence, as with culture, is the need for ...

BACKGROUND There are diverse causes of cicatricial alopecia characterized by lack of follicular ostia and irreversible loss of hair. While clinical differentiation between the causes may be difficult, particularly with regard to lichen planus (LP), lupus erythematosus (LE) and pseudopelade of Brocq (PB), it has been suggested that both histopathologic examination and direct immunofluorescence s...

Abstract Pemphigus vulgaris (PV) is a potentially lethal chronically relapsing autoimmune bullous disorder with intraepithelial lesions affecting the skin and mucous membranes. The prediction of clinical relapse has been studied conventional biopsy plucked hair direct immunofluorescence (DIF; Daneshpazhooh M, Naraghi ZS, Ramezani A et al. Direct for evaluation immunologic remission in PV. J Am ...

females abortion is one of the most important sequela of genital infection with chlamydia trachomatis, mycoplasma hominis and ureaplasma urealyticum.in this study frequency of chlamydia trachomatis, mycoplasma hominis and ureaplasma urealyticum was studied in 125 females with habitual abortion by direct and indirect immunofluorescence tests and culture method and compared with 250 normal popula...

BACKGROUND Leukocytoclastic vasculitis (LCV) is a clinico-pathological entity. Previous direct immunofluorescence study (DIF) studies of vasculitis showed positive findings mainly in the early stage of the disease. OBJECTIVE To study the positive yield and patterns of DIF in patients with various stages of LCV. DESIGN One hundred patients with LCV who attended the Department of Dermatology,...

Herpetic lesions of the genitalia may be confused clinically with other ulcerative, genital lesions. Direct immunofluorescence (FA) provides a rapid method of diagnosis, and the utility of this method for the diagnosis of genital ulcers was examined. One hundred and ten patients with genital lesions were examined by darkfield for syphilis and by FA and culture for herpes simplex virus (HSV) inf...

It is technically impractical for many clinical laboratories to use the direct immunofluorescence assay for identifying and serogrouping clinical isolates of Legionella. We compared the results obtained with the direct immunofluorescence assay with the results of a simple and less-demanding slide agglutination test for identifying 15 serogroups representing seven Legionella species. The slide a...

The diagnosis of toxoplasmic lymphadenitis was established through the demonstration, by direct immunofluorescence, of toxoplasma cysts and trophozoites in a cervical lymph node biopsy which also had a characteristic histopathology. The patient had cervical lymphadenopathy and increased specific fluorescent antitoxoplasma IgG and IgM antibodies.