Acyldihydroxyacetone phosphate was first discovered as a lipid rapidly labelled by ’’Pi or by [p”P]ATP in guinea-pig liver mitochondria (Hajra & Agranoff, 1967,1968). This rapid labelling was due to the dephosphorylation and rephosphorylation of endogenous acyldihydroxyacetone phosphate in mitochondria (Hajra et al., 1968). Acyldihydroxyacetone phosphate was shown to be biosynthesized by the di...

Upon differential centrifugation of rat liver homogenate, the enzyme acyl-CoA:dihydroxyacetone-phosphate acyltransferase (EC 2.3.1.42) was found to be localized in the light mitochondrial (L) fraction which is enriched with lysosomes and peroxisomes. Peroxisomes were separated from lysosomes in a density gradient centrifugation using rats which were injected with Triton WR 1339. By comparing th...

The precise metabolic steps that couple glucose catabolism to insulin secretion in the pancreatic beta cell are incompletely understood. ATP generated from glycolytic metabolism in the cytosol, from mitochondrial metabolism, and/or from the hydrogen shuttles operating between cytosolic and mitochondrial compartments has been implicated as an important coupling factor. To identify the importance...

Electrocatalytic oxidation of glycerol (EOG) is an attractive approach to convert surplus value-added products. Experiments have shown that EOG activity and selectivity depend not only on the electrocatalyst but also electrode potential, pH, electrolyte. For broadly employed gold (Au) electrocatalysts, experiments demonstrated high under alkaline conditions with glyceric acid as a primary produ...

1-0-Alkyl dihydroxyacetone phosphate is biosynthesised in guinea pig liver mitochondria and in rat brain microsomes by direct reaction of a long chain alcohol and acyl dihydroxyacetone phosphate. ATP and Mg* are stimulatory but coenzyme A is not necessary for the reaction. 1-0-Alkyl dihydroxyacetone phosphate (alkyl DHAP)l was shown to be biosynthesized from long chain alcohol and DHAP with ATP...