Tryptic digestion is an important preanalytical step in shotgun proteomics because inadequate or excessive digestion can result in a failed or incomplete experiment. Unfortunately, this step is not routinely monitored before mass spectrometry because methods available for protein digestion monitoring either are time/sample consuming or require expensive equipment. To determine if a colorimetric...

Anaerobic Digestion has become a promising technology for biological transformation of organic fraction of the municipal solid wastes (MSW). In order to represent the kinetic behavior of such biological process and thereby to design a reactor system, development of a mathematical model is essential. Addressing this issue, a simplistic mathematical model has been developed for anaerobic digestio...

The functional response is a key element of predator-prey interactions, and variations in its parameters influence interaction strength and population dynamics. Recent studies have used the equation of the metabolic theory of ecology (MTE) to quantify the effect of temperature on the parameter Th, called "handling time," and then predict the responses of predators and communities to climate cha...

the cane alcohol vinasse assessment on the removal efficiency of a fixed biomass anaerobic reactor (fbar) was evaluated. crude vinasse was used to feed the reactor. the fbar was operated with organic load rate (olr) of 0.5, 1, 3 and 6 gcod/l/d. removal efficiencies of 85, 82, 75 and 66 per cent were observed respectively. the fba reactor presented an excellent removal of organic matter, however...

the bulk of the world's phosphate reserves is located in sedimentary horizons that contain appreciable amounts of carbonate. during beneficiation of phosphate rocks, huge amounts of oversize phosphate rocks are rejected from crushers. this reject also mostly contains low tricalcium phosphate and high carbonate content. in this study, experiments have been carried out to reduce/eliminate carbona...

The present experiment was carried out to determine the digestion kinetics of carbohydrate fractions of citrus by-products. Grapefruit pulp (GP), lemon pulp (LE), lime pulp (LI) and orange pulp (OP) were the test feed. Digestion kinetic of whole citrus by-products and neutral detergent fiber (NDF) fraction and acid detergent fiber (ADF) fractions of citrus by-products were measured using the <e...

Because grain is highly digestible, maize hybrids for silage production are generally chosen based on their The nutritive value of forage maize (Zea mays L.) may be improved grain yield. However, the stalk portion of the maize through genetic selection for increased rate of fiber digestion or decreased indigestible fiber concentration. To identify sources of genetic plant contains 50% or more o...

Tryptic digestion of proteins is one of the critical steps in any proteomics analysis and may heavily impact the quality of the data generated from the sample. The peptides generated are analyzed by mass spectrometry and used to identify and characterize the proteins in the sampled population. Several factors have been found to influence the results of proteolysis and the subsequent analysis. S...

The disposal of excess sludge from wastewater treatment plants is a serious problem that needs to be addressed. Temperature-phased anaerobic digestion (TPAD) which combines thermophilic and mesophilic processes in one, brings together the advantages of both systems. The aim of the present work was to develop a simple kinetic model to describe the TPAD of sewage sludge in batch completely stirre...