Differential display PCR (DD RT-PCR) has been extensively used for analysis of differential gene expression, but continues to be hampered by technical limitations that impair its effectiveness. In order to isolate novel genes co-expressing with human RAG1, we have developed an effective, multi-tiered screening/purification approach which effectively complements the standard DD RT-PCR methodolog...

The formation of intragranular pores, induced by diopside crystallization in diopside–albite glasses, was highlighted in a previous study and here confirmed by SEM observation. The sinter-crystallization kinetics of four glass compositions, labelled G1, G2, G3 and G2-Cr, characterized by the formation of induced crystallisation porosity, PCR, was investigated. The activation energies of crystal...

Differential sensitivity for the release of PCR-detectable genomic DNA upon boiling in water is reported for 45 Campylobacter jejuni and Campylobacter coli strains isolated in Egypt. All of the strains released PCR-detectable DNA when treated with proteinase K and sodium dodecyl sulfate. When DNA was extracted from these strains by boiling in water, nine (20%) of the strains were PCR negative o...

Advances in biotechnology has been the subject of praise for a decade now, this is due to techniques such as gene expression which contributed immensely success genetic engineering, medical advancement, vaccine production and agriculture. Gene become very important tool overall improvement quality life. This paper tries look into development last forty years highlight how technological advancem...

MOTIVATION Polymerase chain reaction (PCR)-based RNA fingerprinting is a powerful tool for the isolation of differentially expressed genes in studies of neoplasia, differentiation or development. Arbitrarily primed RNA fingerprinting is capable of targeting coding regions of genes, as opposed to differential display techniques, which target 3' non-coding cDNA. In order to be of general use and ...

A T-->C point mutation is shown to specifically inhibit PCR amplification when compared to wild-type controls in exon H of the factor IX gene. Multiple primers of different lengths and locations were designed to examine this phenomenon. The experiments suggest that poor annealing and/or extension from the downstream primer are responsible for the observed inhibition and that the mutation can ex...

INTRODUCTION Infectious mononucleosis (IM) caused by the Epstein-Barr virus (EBV) is commonly diagnosed by detection of antibodies in the patient's sera. Differentiation of acute from chronic and differential diagnosis of EBV-induced IM from IM-like syndrome caused by human cytomegalovirus (CMV) is important. The objective of this study was to standardize and use polymerase chain reaction (PCR)...

The host-fungus interaction is characterized by changes in gene expression in both host and pathogen. Differential-display reverse transcription PCR (DDRT-PCR) is a PCR-based method that allows extensive analysis of gene expression among several cell populations. Several limitations and drawbacks to this procedure have now been addressed, including the large number of false-positive results and...

IntroductionAccurate diagnosis of chikungunya (CHIK) is essential for effective disease management and surveillance. In a cohort febrile Congolese patients, available diagnostic methods widely used in CHIK were evaluated. addition, plasma cytokines quantified patients those coinfected with malaria compared healthy controls.MethodsBetween June November 2019, total 107 suspected subjected to diff...

Differential display is a method for the survey, analysis and comparison of gene expression in eukaryotic cells and tissues. Differential display involves isolation of high-quality nondegraded RNA, selective reverse transcription of polyadenylated mRNA using specific anchored oligopolydeoxythymidine [oligo(dT)] primers, and the subsequent PCR amplification of the cDNA with the same oligo(dT), a...