The presence of Cajal-Retzius cells in the adult human prefrontal and visual cortices has been demonstrated with calcium binding protein immunocytochemistry and NADPH-diaphorase histochemistry. These cells expressed parvalbumin, calbindin and calretinin calcium binding proteins and displayed NADPH-diaphorase enzyme activity. The three basic morphological profiles-horizontal, pyriform and multip...

The distribution patterns of NADPH-diaphorase activity in the nucleus olfactorius anterior (NOA) and anterior commissure (AC) of the rat were described using an histochemical technique. In the lateral, dorsal, ventral, medial and posterior subdivisions of the NOA we have demonstrated heavily stained elements (neurons and processes), and slightly labelled cell bodies. The neurons were mainly loc...

The purification and some of the properties of a flavoprotein component of a-ketoglutaric dehydrogenase complex have been described in a previous communication (1). In several of its properties, this flavoprotein shows a remarkable resemblance to the flavoprotein first isolated by Straub (2) and later studied by Savage (3). They have the same prosthetic group (flavin adenine dinucleotide) and a...

DT-diaphorase catalysed the reduction of 1,4-naphthoquinones with hydroxy, methyl, methoxy and glutathionyl substituents at the expense of reducing equivalents from NADPH. The initial rates of quinone reduction did not correlate with either the half-wave reduction potential (E1/2) value (determined by h.p.l.c. with electrochemical detection against an Ag/AgCl reference electrode) or the partiti...

The enzyme isocitrate dehydrogenase (IDH, EC 1.1.1.42) can exhibit activation by one of its products, NADPH. This activation is competitively inhibited by the substrate NADP+, whereas NADPH competes with NADP+ for the catalytic site. Experimental observations briefly presented here have shown that if IDH is coupled to another enzyme, diaphorase (EC 1.8.1.4), which transforms NADPH into NADP+, t...

The neuronal isoform of nitric oxide synthase (nNOS) and soluble guanylate cyclase (sGC) were localized in the cochlea, the cochlear nucleus (CN), and the superior olivary complex (SOC) of Fisher 344 rats. In the cochlea, nNOS was identified in spiral ganglion cells by using nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase histochemistry and in situ hybridization. NADPH-diaphorase...

Previous studies have demonstrated that the epithelium of the respiratory portion of rat nasal mucosa is amply supplied by nerve fibers with immunoreactivities for calcitonin gene-related peptide (CGRP) and substance P (SP), these fibers most likely acting as sensory mediators in the mucosa. The present study demonstrates that some intraepithelial fibers contain a VIP-immunoreactivity whose occ...

The salivary glands of the blood-sucking bug Rhodnius prolixus are formed by a single layer of binucleated epithelial cells surrounded by a double layer of transversely oriented smooth muscle cells. The epithelial cells are rich in rough endoplasmic reticulum and mitochondria and have abundant microvillar projections towards the gland lumen. This cell layer surrounds a relatively large cavity w...