a multiplex pcr-based assay was developed for detection of salmonella typhi and identification of other salmonella serotypes. three primer-sets were selected from different genomic sequences, malo2-f/malo2-ra primers from invasion gene, parat-s/parat-as as well as tyv-s/tyv-as primers from o-antigen gene cluster of the genus salmonella. this method differentiated salmonella spp., based on size ...

background: neisseria meninigitidis is one of the most frequently encountered microorganisms associated with central nervous system infections. the aim of this study was to evaluate a pcr-based assay for specific and rapid detection of n. meninigitidis in csf specimens.   methods : since april 2002 to july 2006, 130 csf specimens were collected from patients suspected of having bacterial mening...

objectives: salmonella typhimurium is important food-borne pathogen responsible for gastroenteritis. in this work a polymerase chain reaction based enzyme linked immunosorbent assay (pcr-elisa) was developed to identify salmonella typhimurium. materials and methods: the rfb gene which is responsible for biosynthesis of the salmonella o-antigenic lipopolysaccharide was selected as the target s...

multiple people detection and tracking is a challenging task in real-world crowded scenes. in this paper, we have presented an online multiple people tracking-by-detection approach with a single camera. we have detected objects with deformable part models and a visual background extractor. in the tracking phase we have used a combination of support vector machine (svm) person-specific classifie...

how to cite this article: karimzadeh p, jafari n, nejad biglari hb, jabbehdari s, alizadeh m, alizadeh gh, nejad biglari hm, sanii s. the clinical features and diagnosis of adrenoleukodystrophy: a case series of iranian family. iran j child neurol. winter 2016; 10(1):61-64. abstract objective adrenoleukodystrophy disorder is one of the x-linked genetic disorders caused by the myelin sheath brea...

objective: this study was designed to investigate rbc indices and hba2 levels in parents of major beta-thalassemia patients to detect possible silent beta- thalassemia carriers and examine its potential impact on the premarital genetic counseling. materials and methods: this cross sectional study was performed at children medical center from 2004 to 2006. after genetic counseling and getting in...

objectives: malaria is the most important tropical disease in terms of morbidity and mortality. the diagnosis of malaria can be conveniently subdivided into clinical, parasitological, biochemical, serological and molecular biological detection. the objective of the present work was to compare two techniques, blood smear and pcr-rflp, for detection of plasmodium species. materials & method...

conclusions the results prove that the presented double pcr method could be used to detect positive cases from culture-negative blood samples. the chelex 100 method is simpler and safer than the use of qiagen kit for dna extraction. materials and methods a total of 100 patients with suspected brucellosis were included in this experimental study and given positive serological tests. diagnosis wa...

in rabbits, especially in some races, limb movement disorder called splay legs, are mostly unilateral or bilateral in front or hind legs at an early age. the severity ranges from mild to severe in this case. in breeding colony of albino dutch laboratory rabbits, the splay leg disorder has been observed over the years permanently.within the scope of this research, 1.5-2.5% of 700-750 number of c...

the problem of eye detection in face images is very important for a large number of applications ranging from face recognition to gaze tracking. in this paper we propose a new algorithm for eyes detection. first, the face region is extracted from the image by skin-color information. second, horizontal projection in image is used to approximate region of the eye be obtained . at last, the eye ce...