The poultry red mite (PRM) Dermanyssus gallinae is well known for its vectorial role pathogens, such as Salmonella enterica ser. Gallinarum, the causative agent of fowl typhoid. Here, we ascertained vertical transmission S. Gallinarum across PRM life stages, combining Baudruche-based in vitro feeding system and a PRM-fitting DNA extraction detection method by qPCR. Small-sized pools (4–5 specim...