Because creatine kinase (CK) appears in cardiac lymph after myocardial infarction, this study was undertaken to determine whether lymph inactivates CK in vitro and whether interruption of cardiac lymph flow influences estimation of infarct size based on plasma CK changes in conscious dogs. After the effects of incubation of canine myocardial CK in native, deproteinized, or sulfhydrylfortified l...

In their report that nearly 50% of healthy young Canadian adults have subnormal serum vitamin C concentrations, Cahill et al. (1) cite our observation that 60% of the acute-care patients in a tertiary care Montreal hospital had subnormal plasma vitamin C concentrations (2). More pertinently, we also observed that only 13% of people concurrently attending the hospital’s outpatient test center ha...

In 1926 Campbell and Hanna (1) published a method for the determination of fructose based upon the reduction of molybdenum in phosphoric acid solution and the reoxidation of the reduced molybdenum with potassium permanganate. This method is not entirely specific for fructose; other substances in blood and urine have some reducing action upon the molybdic acid reagent. The method will not permit...

In patients with renal insufficiency, the elevated plasma nonprotein nitrogen cannot be accounted for solely by the rise in the urea nitrogen (2). It is known that other nitrogenous materials such as creatinine and uric acid contribute to this elevation. Since free amino acids also contribute to the nonprotein, nonurea nitrogen of plasma, several investigators have determined the amino acid com...

The polyadenylic acid-containing messenger ribonucleic acids of eukaryotic cells are rapidly isolated and deproteinized in a simple and gentle one-step procedure. The polyribosome fraction, dissolved in 0.5 M NaCl/0.5 percent sodium dodecyl sulfate, is passed through an oligo(dT)-cellulose column which is then washed with the solvent until proteins and contaminating ribonucleic acids are fully ...

OBJECTIVE To assess the efficacy of platelet rich plasma (PRP) in the treatment of extensive corneal ulcers in albino rabbits. METHODS New Zealand rabbits, divided in 3 groups, were used for the study. Corneal ulcers of 10mm diameter were made. Rabbits blood was extracted for the preparation of the PRP of the corresponding group. The blood was processed by differential centrifugation. The fir...

Background. At present, for a number of reasons the complete bone defect replacement with autogenous is not always possible. Bone substitute materials are used as an alternative to tissue and can be either biological or non-biological origin. One ways development reconstructive technologies use tissue-engineered constructs that fully imitate in required volume.
 Aim study define vivo possi...

The present study examined the impact of the supplementation of glutathione (GSH), γ-L-glutamyl-L-cysteinyl-glycine, on human blood GSH levels. Healthy human volunteers were orally supplemented with GSH (50 mg/kg body weight). Venous blood was collected from the cubital vein before and after ingestion. Plasma was mixed with 3 volumes of ethanol. The supernatant and precipitate were used for the...

The in vivo binding of nickel to chromatin, nucleic acids, and nuclear proteins from rat kidney and liver was investigated. Evidence is presented for the direct interaction of nickel with chromatin from rat tissues following i.p. injection of nickel carbonate. A gentle DNA isolation procedure was developed and used to isolate nickel-bound whole chromatin, DNA + histone octamer complex (polynucl...