نتایج جستجو برای: dependent probe amplifi cation
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I nflammatory breast cancer (IBC) is a rare form of the disease that differs substantially from noninflammatory breast cancer in presentation and response to therapy. Despite those differences, researchers have uncovered few molecular characteristics that distinguish IBC from noninflammatory breast cancer, and IBC treatment generally relies on standard breast cancer regimens. Cancer Center in H...
EGF receptor (EGFR) –mutant lung cancers eventually become resistant to treatment with EGFR tyrosine kinase inhibitors (TKI). The combination of EGFR-TKI afatinib and anti-EGFR antibody cetuximab can overcome acquired resistance in mouse models and human patients. Because afatinib is also a potent HER2 inhibitor, we investigated the role of HER2 in EGFR-mutant tumor cells. We show in vitro and ...
Introduction. Polymerase chain reaction (PCR)-based diagnostic tests use purifi ed nucleic acids (NAs) from clinical samples. The NAs cation step adds time, cost, and aff ects the quality of testing. objective this study was to develop a protocol for direct saliva in genetic markers, without acids. Methods. PCR, real-time RT-PCR isothermal amplifi were used detection Results. We report markers ...
The cation-complexing carboxylic-acid antibiotic X-537A, at concentrations far below that required for ionophorous activity, selectively inhibits the oxidation of glutamate and isocitrate by liver mitochondria in steady-state 3. The site of inhibition has been localized specifically at the reduction of NADP(+). Glutamate and isocitrate dehydrogenases, the oxidation of NAD(+)-dependent substrate...
identifi cation of mycobacteria by DNA amplifi cation and oligonucleotide-specifi c capture plate hybridization. 16S rRNA sequence diversity in Myco-bacterium celatum strains caused by presence of two different copies of 16S rRNA gene.ment of partial sequencing of the 65-ki-loDalton heat shock protein gene (hsp65) for routine identifi cation of Mycobacteri-um species isolated from clinical sour...
First-principle calculations are employed to investigate the ultrafast isomerization of acetylene cation and dication. We use time-dependent density functional theory together with Ehrenfest dynamics track coupled electron-nuclear dynamics. For both dication, we observe nonadiabatic behaviors during isomerization. find that charge transfer not only alters electronic structure through transition...
Vibrational Stark shifts were explored in aqueous solutions of organic molecules with carbonyl- and nitrile-containing constituents. In many cases, the vibrational resonances from these moieties shifted toward lower frequency as salt was introduced into solution. This is contrast to blue-shift that would be expected based upon Onsager's reaction field theory. Salts containing well-hydrated cati...
رشد و گسترش بسیاری از گیاهان به واسطه فعالیت هورمون های گیاهی تنظیم می شود. نتایج تجربی نشان دادند که هورمون تراپی با هورمون ga3 تاثیر معناداری بر روی توزیع مونوترپن ها در گیاهrubus caesius دارد. در حضور هورمون ga3 غلظتcineole و ? –cadinene کاهش و میزان ? –terpinoleneand longifoleneافزایش یافت. با بررسی مسیر بیوسنتزی مشخص شد که ? –terpinolene و cineoleاز حدواسط ? –terpinyl cation مشتق شده اند و...
The potential advantages of using the California Consonant Test (CCT) (Owens & Schubert, 1977) with children are discussed, followed by reports of two exploratory investigations. In the fi rst investigation, the CCT was administered in classrooms to second, third and fourth graders with normal hearing. The children scored within 13% of an adult control group, suggesting that the CCT was not too...
February 2006 | Volume 3 | Issue 2 | e99 Now that the media frenzy over West Nile virus (WNV) has subsided, and pictures of dead birds and insect repellant cans no longer permeate the nightly news, what shall we do about screening the United States blood supply for the virus? Shortly after the spread of WNV in North America and the revelation that blood transfusions may transmit the virus [1], ...
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