The relationship between thyroid plasma membrane phosphorylation and thyrotropin (TSH) receptor degradation was investigated by using bovine thyroid tissues. By fractionation of thyroid cytosol (105,000 X g supernatant of thyroid homogenate) in a continuous sucrose density gradient centrifugation, three different TSH binding activities were separated. During the incubation of thyroid plasma mem...

High-buoyant-density proteoglycan aggregates could not be prepared from extracts of adult human cartilage by associative CsCl-density-gradient centrifugation with a starting density of 1.68 g/ml, even though proteoglycan subunits, hyaluronic acid and link proteins were all present. In contrast, aggregates could be prepared when extracts of neonatal human cartilage or bovine nasal cartilage were...

To identify previously unknown peroxisomal proteins, we established an optimized method for isolating highly purified peroxisomes from etiolated soybean cotyledons using Percoll density gradient centrifugation followed by iodixanol density gradient centrifugation. Proteins in highly purified peroxisomes were separated by two-dimensional PAGE. We performed peptide mass fingerprinting of proteins...

Rat cerebral cortex was homogenized in a sucrose-free medium and a number of intracellular melnbrane fractions were isolated therefrom by means of differential and density-gradient centrifugation. The mitochondrial and microsomal membrane fractions, as well as the separated rough and smooth endoplasmic reticulum components of the latter, were subjected to zonal density gradient electrophoresis....

Lymphocytes were separated according to their buoyant density, by centrifugation to equilibrium in continuous gradients of albumin. The procedure gave good resolution, high reproducibility, and good recovery of biologically active cells. Lymphocytes were separable into a series of discrete density subpopulations. [The SCI indicates that this paper has been cited over 185 times since 1968.]