The key principle of gene delivery to articulations by direct intra-articular injection is to release complementary DNA(cDNA)-encoding medical products that will lead to maintained, endogenous production of the gene products withinthe articulation. In fact, this has been accomplished for both in vivo and ex vivo gene delivery, using several vectors,genes, and cells in some animal models. Some c...

Objective(s): The interaction of DNA with iron oxide nanoparticles (SPIONs) was studied to find out the interaction mechanism and design new drug delivery systems. Materials and Methods: The interaction of calf thymus DNA (ctDNA) with SPIONs doped with 2H-chromene via dopamine as cross linker (SPIONs@DA-Chr) was studied using the UV absorption spectroscopy, viscosity measurement, circular dichr...

background: world health organization (who) defines three goals to assess the performance of a health system: the state of health, fairness in financial contribution and responsiveness. we assessed the responsiveness of health system for patients with diabetes in a defined population cohort in tehran, iran. methods: total responsiveness and eight domains (prompt attention, dignity, communicatio...

background & aim: postpartum period is accompanied by significant changes in women’s quality of life. these alterations can affect the health of mothers and children. considering the importance of postnatal quality of life and its different contributing factors, this study aimed to compare women’s quality of life after vaginal delivery and cesarean section. methods:this retrospective cohort stu...

receiver operating characteristics (roc) curves have numerous applications for identifying a cut-off point in diagnostic tests. nonetheless, given that sometimes two cut-off points have to be specified simultaneously, the roc curve can be used to identify such points. the volume under the roc surface (vus) serves as a criterion for the accuracy of diagnostic tests. one of the unfortunate outcom...

Use of non-biological agents for mRNA delivery into living systems in order to induce heterologous expression of functional proteins may provide more advantages than the use of DNA and/or biological vectors for delivery. However, the low efficiency of mRNA delivery into live animals, using non-biological systems, has hampered the use of mRNA as a therapeutic molecule. Here, we show that gold na...

Biodegradable poly(ester amine) (PEA)-based and poly(amido amine) (PAA)-based nanoparticles were developed for efficient in vitro siRNA delivery to human umbilical vein endothelial cells (HUVECs). They were screened, characterized, and compared with traditionally studied DNA-containing particles. Several of the polymeric nanoparticles tested were found to be effective for delivering functional ...

A foundational element of modern healthcare is an evidence-based practice. However, the conduct of research to generate evidence and the subsequent application of research findings are viewed by many Canadian healthcare organizations as separate from healthcare delivery. This mindset impedes effective translation of knowledge into practice. In this article, underlying issues that enable this di...