نتایج جستجو برای: degenerate pcr
تعداد نتایج: 195264 فیلتر نتایج به سال:
5′-Degenerate 3′-Dideoxy-Terminated Competitors of PCR Primers Increase Specificity of Amplification
Carbon nanoscrolls (CNSs) with tubular structure similar to the open multiwall carbonnanotube have been of hot debate during recent years. Due to its unique property, Graphene Nanoscroll (GNS) have attracted many research groups’ attention and have been used by them. They specially studied on energy storage devices such as batteries and super capacitors. These devices can be schematically...
it is well known that in operations research, degeneracy can cause a cycle in a network simplex algorithm which can be prevented by maintaining strong feasible bases in each pivot. also, in a network consists of n arcs and m nodes, not considering any new conditions on the entering variable, the upper bound of consecutive degenerate pivots is equal $left( begin{array}{c} n...
Degenerate kernel approximation method is generalized to solve Hammerstein system of Fredholm integral equations of the second kind. This method approximates the system of integral equations by constructing degenerate kernel approximations and then the problem is reduced to the solution of a system of algebraic equations. Convergence analysis is investigated and on some test problems, the propo...
A rapid, sensitive and highly specific detection method for Aquareovirus based on reverse-transcription polymerase chain reaction (RT-PCR) was developed. Based on multiple sequence alignment of the cloned sequences of a local isolates, the Threadfin reovirus (TFV) and Guppy reovirus (GPV) with Grass carp reovirus (GCRV), a pair of degenerate primers was selected carefully and synthesized. Using...
PCR amplification using COnsensus DEgenerate Hybrid Oligonucleotide Primers (CODEHOPs) has proven to be highly effective for identifying unknown pathogens and characterizing novel genes. We describe iCODEHOP; a new interactive web application that simplifies the process of designing and selecting CODEHOPs from multiply-aligned protein sequences. iCODEHOP intelligently guides the user through th...
Staphylococcal enterotoxins (SEs) are a family of 17 major serological types of heat-stable enterotoxins that are one of the leading causes of gastroenteritis resulting from consumption of contaminated food. SEs are considered potential bioweapons. Many Staphylococcus aureus isolates contain multiple SEs. Because of the large number of SEs, serological typing and PCR typing are laborious and ti...
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