Orthodontic clear aligner treatment is gaining tremendous popularity. The world market leader Align Technology® and its product Invisalign®. Although numerous patients are treated with Invisalign® aligners, only little known about the cellular effects of material on oral epithelial cells. In present study, we aimed to investigate SmartTrack® directly cultured primary human keratinocytes (HOKs)....

The Microscale Life Sciences Center desires an automated count of fluorescing live/dead biological cells from images obtained via microscopy. Various cell counting procedures were explored in the literature. Four methods were tried: threshold segmentation, watershed segmentation, crosscorrelation, and a threshold “real-time” cross-correlation hybrid. The threshold technique produced the best re...

proton exchange membrane fuel cells (pemfcs) with a dead-ended anode and cathode can obtain high hydrogen and oxygen utilization by a comparatively simple system. nevertheless, the accumulation of the water in the anode and cathode channels might cause a local fuel starvation degrading the performance and durability of pemfcs. in this study, a brand new design for a polymer electrolyte membrane...

"Air-seeding" is a proposed mechanism for the initiation of water stress embolism in dead plant cells. During air-seeding, external air is drawn into the lumen of a dead plant cell through a pore or crack in the cell wall. The resulting bubble may expand to fill the lumen, thus embolizing the cell. The data presented confirm that Sphagnum hyalocysts can embolize by air-seeding when the pressure...

RNA helicases of the DEAD-box family are involved in essentially all RNA-dependent cellular processes. In this issue of Cell, Sengoku et al. (2006) solve the structure of the DEAD-box protein Vasa in the presence of RNA and a nonhydrolyzable ATP analog and provide important insights into how this family of helicases unwinds RNA.

DNGR-1 (CLEC9A) is a receptor for necrotic cells required by DCs to cross-prime CTLs against dead cell antigens in mice. It is currently unknown how DNGR-1 couples dead cell recognition to cross-priming. Here we found that DNGR-1 did not mediate DC activation by dead cells but rather diverted necrotic cell cargo into a recycling endosomal compartment, favoring cross-presentation to CD8(+) T cel...

AIM To evaluate a flow cytometry protocol that uses reference beads for the enumeration of live and dead bacteria present in a mixture. METHODS AND RESULTS Mixtures of live and dead Escherichia coli with live:dead concentration ratios varying from 0 to 100% were prepared. These samples were stained using SYTO 9 and propidium iodide and 6-μm reference beads were added. Bacteria present in live...

Escherichia coli contains five members of the DEAD-box RNA helicase family, a ubiquitous class of proteins characterized by their ability to unwind RNA duplexes. Although four of these proteins have been implicated in RNA turnover or ribosome biogenesis, no cellular function for the RhlE DEAD-box protein has been described as yet. During an analysis of the cold-sensitive growth defect of a stra...

DEAD-box RNA helicases, defined by the sequence Asp-Glu-Ala-Asp (DEAD, in single-letter amino-acid code), regulate RNA unwinding and secondary structure in an ATP-dependent manner in vitro [1] and control mRNA stability and protein translation. Both yeast and mammals have large families of DEAD-box proteins, many of unknown function. We have disrupted a Dictyostelium discoideum gene, helC, whic...

knowledge of the stand condition and the gradual replacement of tree species in different development stages and phases provide appropriate information related to the close to nature treatments. stem exclusion phase is one of the important development phases in the forest stands development. the aim of study was to analyze the characteristics of dead trees in the stem exclusion phase in the les...