OBJECTIVE To screen and analyze key express sequence tags (ESTs) which were differentially displayed in every period of SD rats' primary hepatic carcinoma and reveal the molecular mechanism of carcinogenesis. METHODS Using diethylnitrosamine (DENA) as a cancerigenic agent, animal models with different phases of primary hepatic cancer were constructed in SD rats. Rats were respectively sacrifi...

In order to understand the molecular basis of chicken heterosis in reproduction traits, mRNA differential display (DDRT-PCR) methods were used to analyze the differential gene expression of ovary tissue between hybrids and their parental lines in a 4×4 diallel cross, involving 4 chicken breeds, which were White Plymouth Rock (E), CAU Brown (D), Silkies (C) and White Leghorn (A). Total of 331 di...

Primary mediastinal large B-cell lymphoma (PMBL) appears to be a distinct clinicopathologic entity among diffuse large B-cell lymphomas (DLBLs). To find molecular alterations associated with this disease, we compared the mRNAs expressed in 3 PMBLs and 3 peripheral DLBLs by differential display-reverse transcription (DDRT) and identified a mRNA specifically expressed in PMBLs. Sequence analysis ...

Causes of maternal mortality are bleeding, hypertension, infection, prolonged labor, and abortion. Bleeding is the highest cause death while labor lowest mortality. Other factors such as condition pregnant women with heart disease, tuberculosis or other diseases suffered by mother. This community service aims to Empower Posyandu Cadres through Increased Knowledge High-Risk Early Detection Pregn...

Academic dissertation To be presented for public criticism, with permission of the Abbreviations ABA abscisic acid HRGP hydroxyproline-rich glycopro-tein AOX alternative oxidase JA jasmonic acid APX ascorbate peroxidase MJ methyl jasmonate ARE antioxidant responsive element MPT1 mitochondrial phosphate translocator1 CAT catalase MRE metal responsive element Cat1AS CAT1 antisense mRNA messenger ...

Establishment of a plant-pathogen interaction involves differential gene expression in both organisms. In order to isolate Botrytis cinerea genes whose expression is induced during its interaction with tomato, a comparative analysis of the expression pattern of the fungus in planta with its expression pattern during in vitro culture was performed by differential display of mRNA (DDRT-PCR). Disc...

In an effort to characterize molecular events contributing to lineage commitment and terminal differentiation of stem/progenitor cells, we have used differential display reverse transcription polymerase chain reaction (DDRT-PCR) and cell lines blocked at two distinct stages of differentiation. The cell lines used were EML, which is representative of normal multipotential primitive progenitors (...

In the era of the Human Genome Project, quantitation of gene expression by tumor/host cells is of paramount importance to investigate gene patterns responsible for cancer development, progression and response/resistance to treatment. Quantitative real-time PCR (qrt-PCR) technology has recently reached a level of sensitivity, accuracy and practical ease that support its use as a routine bioinstr...

Evaluation of in vivo gene expression in specific types of cells is difficult because animal tissues are complex mixtures of many cell types, and RNA extracted from homogenized fresh whole tissue is derived from all cell types present. In an attempt to improve specificity, RNA has been isolated from specific cells microdissected from fixed, paraffin-embedded tissue sections (2,6), but the yield...

Amplified fragment length polymorphism (AFLP) and enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) genomic fingerprinting assays were compared for their ability to differentiate Escherichia coli isolates obtained from various host sources, and with respect to their pathogenicity. One hundred and ten verotoxigenic, enterotoxigenic and non-pathogenic E. coli is...