Semi-conservative DNA synthesis reactions catalyzed by the bacteriophage T4 DNA polymerase holoenzyme are initiated by a strand displacement mechanism requiring gp32, the T4 single-stranded DNA (ssDNA)-binding protein, to sequester the displaced strand. After initiation, DNA helicase acquisition by the nascent replication fork leads to a dramatic increase in the rate and processivity of leading...

Doering, Alice McGovern (University of Pennsylvania School of Medicine, Philadelphia), Miekie Jansen, and Seymour S. Cohen. Polymer synthesis in killed bacteria: lethality of 2',3'-dideoxyadenosine. J. Bacteriol. 92:565-574. 1966.-We studied the metabolic capabilities of cells that had lost the ability to multiply under a variety of lethal treatments. Cultures of a polyauxotrophic mutant of Esc...

OBJECTIVE People with dually diagnosed substance abuse and mental illnesses often feel alienated at traditional 12-step meetings, yet they need the peer support provided by such groups. Dual Diagnosis Anonymous (DDA) is a peer-support program specifically for people with co-occurring disorders, which addresses many of the factors that members find alienating about traditional 12-step groups. Th...

A convenient delay, Doppler and angular-(DDA) domain representation of the multiple-input multiple-output (MIMO) wireless channel is conceived for deriving end to relationship in delay-Doppler (DD)-domain orthogonal time frequency space (OTFS)-based communications. Subsequently, a time-domain pilot based model developed estimating DDA-domain state information (CSI) our MIMO OTFS system. The key...

We have studied the effect of chitosan sponges, produced from batches with distinct degree deacetylation (DDA) and molecular weight (Mw), on adhesion, growth differentiation primary human osteoblasts an aim to offer a suitable tool for guided bone regeneration. All sponges revealed similar microstructure, irrespective DDA (58, 73, 82, 88, 91 %) Mw (749, 547, 263, 215, 170 kDa, respectively). Ce...

Pickering emulsions (PEs) have recently gained increasing attention as green carriers of bioactive agents due to their surfactant-free and eco-friendly nature. Herein, the effect degree deacetylation (DDA) chitosan on properties chitosan/gum Arabic (CH/GA) particles PEs was investigated. CH/GA with a DDA 96% (high DDA) 78% (low were prepared. The contact angle water drop for prepared high low 8...

Dynamic difficulty adjustment (DDA) is a technique for adaptively changing a game to make it easier or harder. A common paradigm to achieve DDA is through heuristic prediction and intervention, adjusting game difficulty once undesirable player states (e.g., boredom or frustration) are observed. Without quantitative objectives, it is impossible to optimize the strength of intervention and achiev...

Diverse Discrete Discriminant Analysis (DDA) models perform differently on different sample observations (Brito et al.(2006)). This fact has encouraged research in combined models for DDA. This research seems to be specially promising when the a priori classes are not well separated or when small or moderate sized samples are considered, which often occurs in practice. In this work we evaluate ...

There are two widely accepted restrictions on the application of the discrete dipole approximation (DDA) in the study of light scattering by particles comparable to the wavelength: (1) when considering dielectric particles, the size of the cells must satisfy the condition kd|m|<0.5, where k is the wavenumber, d is the size of the cells, and m is the complex refractive index of the constituent m...

The formation of marine snow (MS) by the toxic diatom Pseudo-nitschia australis was simulated using a roller table experiment. Concentrations of particulate and dissolved domoic acid (pDA and dDA) differed significantly among exponential phase and MS formation under simulated near surface conditions (16 C/ 12:12-dark:light cycle) and also differed compared to subsequent particle decomposition a...