BACKGROUND In order to study the anticancer effects and cellular apoptosis pathways induced by daidzein. MATERIALS AND METHODS We used the human MCF-7 breast cancer cell line as a model and examined the apoptosis by Hoechst-propidium iodide staining fluorescence imaging and flow cytometry. RESULTS Our data indicated that daidzein induces antiproliferative effects in a concentration- and tim...

OBJECTIVES This paper reported the effects of commonly used whole soy foods (soy flour) and purified daidzein (one of the major isoflavones and the precursor of equol) on changes in anthropometric measurements and body composition in a 6-month double-blind, randomized, placebo-controlled trial among prehypertensive postmenopausal women who are also equol producers. METHODS 270 eligible women ...

BACKGROUND Radix Puerariae (Gegen) contains abundant isoflavones in the forms of glycosides and aglycones, such as daidzein, daidzin and puerarin. This study aims to investigate the intestinal absorbability and mechanism of these three structurally related isoflavones. METHODS The bi-directional transport of these three isoflavones in Caco-2 monolayer model was performed to evaluate their abs...

This study examined the effects of rice bran oil (RBO) on mouse intestinal microbiota and urinary isoflavonoids. Dietary RBO affects intestinal cholesterol absorption. Intestinal microbiota seem to play an important role in isoflavone metabolism. We hypothesized that dietary RBO changes the metabolism of isoflavonoids and intestinal microbiota in mice. Male mice were randomly divided into two g...

The phytoestrogens genistein, daidzein and the daidzein metabolite equol have been shown previously to possess oestrogen agonist activity. However, following consumption of soya diets, they are found in the body not only as aglycones but also as metabolites conjugated at their 4'- and 7-hydroxyl groups with sulphate. This paper describes the effects of monosulphation on the oestrogen agonist pr...

Background: Previous studies show that fermentation using Aspergillus oryzae and Rhizopus oligopsorus increases the aglyconic isoflavone in soybean with highest content reached on seventh day. Still, no research has been done edamame. Objective: This study was conducted to validate method determine isoflavone, daidzein, non-fermented A. R. fermented edamame TLC densitometry. Method: densitometr...

Mechanism of chemoprevention by daidzein (500 μg/g bwt) was examined by injecting it subcutaneously at 16th, 18th, and 20th day postpartum, followed by counting of terminal end buds (TEBs), terminal ducts (TDs), and lobules and immunohistochemistry of ER-α, Bcl2, Bax, and caspase-3. DNA fragmentation was also analysed to measure the apoptosis level. Estradiol benzoate (EB) (500 ng/g bwt) and di...

The amount of new bone formed in collagen matrix with daidzein was compared to that formed in collagen matrix alone. Eighteen bone defects, 5mm by 10mm were created in parietal bone of 9 New Zealand white rabbits. In the experimental group, 6 defects were grafted with collagen matrix with daidzein. In the control groups, 6 defects were grafted with collagen matrix alone (positive control) and 6...

Genistein and daidzein are biologically active isoflavones that are especially abundant in soybeans. After intestinal absorption, circulating genistein and daidzein are eliminated primarily by the kidneys. This study was undertaken to assess the metabolism of genistein and daidzein in patients with end-stage renal disease (ESRD) on hemodialysis therapy, and to test whether this treatment modali...

Objective: The present study was designed to investigate the immunomodulatory potential of Pueraria tuberosa tuber extract and isoflavones puerarin, daidzein and genistein in rats. Methods: The immunomodulatory potential of standardized P. tuberosa tuber extract (100-200 mg/kg; p.o.) and isoflavones puerarin (25-50 mg/kg; p.o.), daidzein (25-50 mg/kg; p.o.) and genistein (25-50 mg/kg; p.o.) was...