An indirect immunofluorescence technique, using the globulin fraction of rabbit antihuman gastrin serum, was applied to formalin-fixed material obtained by suction biopsy from the fundic mucosa of nine cases of pernicious anaemia. Cytochemical tests for endocrine polypeptide cells of the APUD series, in which the G cell is included, were carried out in parallel with immunofluorescence and with ...

The cytochemical localization of G6Pase activity, which is specific to the endoplasmic reticulum (ER) of Müller cells, was studied after argon laser photocoagulation in the guinea pig retina. After argon laser radiation, Müller cells exhibited enlargement of the cytoplasm, an increase of reactive ER and the nuclei, dislocation of the nuclei and diagonal stretching of the cytoplasm. However, cel...

Proper fixation of lung tissue for morphological and cytochemical studies by transmission electron microscopy (TEM) is difficult by conventional (perfusion and immersion) fixation processes. A broiler model (Gallus domesticus) of pulmonary hypertension was the source of lungs for this study. Microwave and intermittent vacuum were used in order to have better structural and enzymatic preservatio...

Although Golgi elements were first obtained from rat liver homogenates as a single fraction (1, 2) a new procedure was recently developed (3) which yielded three fractions which accounted for all of the Golgi elements present in the microsomal fraction, as indicated by recovery of UDPgalactosyl transferasel (4) . Morphological analysis showed that the Golgi "light" and "intermediate" subfractio...