The antibiotic cycloheximide inhibits chain initiation and the extension of nascent protein chains (6). One might therefore expect cycloheximide to have a number of indirect effects depending on the duration of the treatment and the concentrations used. For example, in several in vivo systems cycloheximide has been shown to block protein synthesis completely at concentrations of 5 to 10 ,ug/ml ...

Mature, self-incompatible stigmas exposed to cycloheximide for 2 h prior to pollination supported identical germination and growth of both cross and self pollen. Treatment of self-pollinated pistils with cycloheximide resulted in the germination of hitherto inactive pollen after some 2-4 h. Pollen germination and initial tube growth in an in vitro germination medium were not significantly affec...

Although the total growth of both zoopathogenic and saprophytic fungi on cycloheximide media was consistently less than that on the control without the antibiotic, a progressive increase in the growth rate of these organisms occurred during exposure to the drug. The extent of this change depended upon the concentration of cycloheximide, the species and strain of the test organism, and the durat...

Protein synthesis by intact Bartlett pear fruits was studied with ripening as measured by flesh softening, chlorophyll degradation, respiration, ethylene synthesis, and malic enzyme activity. Protein synthesis is required for normal ripening, and the proteins synthesized early in the ripening process are, in fact, enzymes required for ripening. (14)C-Phenylalanine is differentially incorporated...

A large number of genes control growth of the yeast Saccharomyces cerevisiae at low temperatures (< 10 degrees ). Approximately 47 percent of the mutants selected for inability to grow at 4-5 degrees C show increased sensitivity to cycloheximide. In 3 of 4 cases tested, supersensitivity to cycloheximide and inability to grow at the low temperature segregate together and thus appear to be effect...

The effect of sequence and timing of hyperthermia (43 degrees) and bleomycin on Chinese hamster cells (V79) has been investigated. Hyperthermia preceding bleomycin treatment produced a greater cytotoxic effect than bleomycin treatment preceding hyperthermia. Furthermore, it appears that the combination of hyperthermia and bleomycin becomes less effective if the application of bleomycin is delay...

1. The liver ribosomes of rats given cycloheximide by intraperitoneal injection incorporate less amino acid into protein than ribosomes from control rat liver when they are incubated in vitro with excess of Sephadex-treated cell sap. The effect is rapid, marked and persistent. 2. Cell sap from liver of cycloheximide-treated animals is inhibitory but the inhibition can be relieved almost entirel...