The channels belonging to the KCNQ gene family consist of 5 different subtypes, which assemble as pentameric channels. The KCNQ2-5 subunits are highly expressed in the ventrobasal thalamus (VB) where they function primarily as KCNQ2/3 heteromers. They underlie an outward potassium (K+)-current, called M-current (IM), which provides a hyperpolarizing drive, thus regulating neuronal excitability....

introduction: the ionic currents show nonlinear behavior and electrical stimuli can influence their activation and inactivation kinetics. the time constant of depolarizing or hyperpolarizing current pulses has an important role in the firing behavior of neurons. in the present study, the effects of quasitrpezoidal command potentials on l-type calcium channel of somata of f1 neuron in helix aspe...

OBJECTIVE Electrophysiological recordings of single neurons in brain tissues are very common in neuroscience. Glass microelectrodes filled with an electrolyte are used to impale the cell membrane in order to record the membrane potential or to inject current. Their high resistance induces a high voltage drop when passing current and it is essential to correct the voltage measurements. In partic...

The whole-cell, patch clamp [corrected] method was applied to olfactory receptor cells in slice preparations made from bullfrog olfactory epithelium. Under voltage-clamp conditions, olfactory receptor cells showed a transient inward current followed by a steady outward current in response to depolarizing voltage steps, as has been shown in the isolated preparation. The input resistance was 5.4 ...

The presence of a hyperpolarization-activated inward current (Ih) was investigated in mouse vestibular primary neurons using the whole-cell patch-clamp technique. In current-clamp configuration, injection of hyperpolarizing currents induced variations of membrane voltage with prominent time-dependent rectification increasing with current amplitudes. This effect was abolished by 2 mM Cs+ or 100 ...

A novel ZVZCS isolated dual series-resonant active-clamp dc–dc converter is proposed to obtain high efficiency. The proposed converter employs an active-clamp technique, while a series-resonant scheme controls the output voltage with the complementary pulse width modulation controller. The active-clamp circuit serves to recycle the energy stored in the leakage inductance or the magnetizing indu...

There are a growing number of current clamp meters and current clamp accessory devices appearing in the calibration workload capable of measuring currents up to 1000 Amps AC and DC. In the past users have attempted to make their own current coils to enable calibration sources with 10A outputs to provide stimuli adequate to calibrate these devices close to their full scale values, often without ...

The mushroom body of the insect brain is an important locus for olfactory information processing and associative learning. The present study investigated the biophysical properties of Kenyon cells, which form the mushroom body. Current- and voltage-clamp analyses were performed on cultured Kenyon cells from honeybees. Current-clamp analyses indicated that Kenyon cells did not spike spontaneousl...