OBJECTIVES Recombinant protein production processes in Escherichia coli are usually operated in fed-batch mode; therefore, the elaboration of a fed-batch cultivation protocol in microtiter plates that allows for screening under production like conditions is particularly appealing. RESULTS A highly reproducible fed-batch like microtiter plate cultivation protocol for E. coli in a micro-bioreac...

In the current investigation, evaporation and transpiration potential were also estimated by means of FAO Penman- Monteith evapotranspiration estimation method and Deviation from Optimum Percentage (DOP) technique by climatic elements (min-max temperature, relative humidity, wind speed and radiation) during at least 10 years statistical period regarding sunflower phenological conditions in Kurd...

The aim of this work was to study the physiological changes of immobilized brewing yeast Saccharomyces cerevisiae in a continuous reactor. Continuous cultivation was carried out at three different temperatures (15, 20 and 25°C) with an industrial brewing yeast strain (UNICER, Bebidas de Portugal, S.A.). During the continuous experiment the viability of free and immobilized cells was followed us...

Pichia pastoris is a prominent host for recombinant protein production, amongst other things due to its capability of glycosylation. However, N-linked glycans on recombinant proteins get hypermannosylated, causing problems in subsequent unit operations and medical applications. Hypermannosylation is triggered by an α-1,6-mannosyltransferase called OCH1. In a recent study, we knocked out OCH1 in...

Strawberry bacterial angular leaf spot (ALS) disease, caused by Xanthomonas fragariae has become increasingly problematic in the strawberry agro-industry. ALS causes small angular water-soaked lesions to develop on the abaxial leaf surface. Studies reported optimum temperature conditions for X. fragariae are 20°C and the pathogen suffers mortality above 32°C. However, at the nursery stage, dise...

PURPOSE The bacterial cultivation conditions for obtaining anti-TNF-α single chain variable fragment (scFv) antibody as the soluble product in E. coli was investigated. METHODS To avoid the production of inclusion bodies, the effects of lactose, IPTG, incubation time, temperature, shaking protocol, medium additives (Mg+2, sucrose), pH, osmotic and heat shocks were examined. Samples from bacte...

A major portion of the over expressed yeast mitochondrial aconitase, a large 82 kDa monomeric TCA cycle enzyme, in Escherichia coli led to the formation of inclusion bodies. Bacterial chaperonin GroEL mediated the correct folding of aconitase with the assistance of its co-chaperonin GroES in an ATP dependent manner. Till date the chaperonin assisted folding of aconitase was limited to the shake...

In the current investigation, evaporation and transpiration potential were also estimated by means of FAO Penman- Monteith evapotranspiration estimation method and Deviation from Optimum Percentage (DOP) technique by climatic elements (min-max temperature, relative humidity, wind speed and radiation) during at least 10 years statistical period regarding sunflower phenological conditions in Kurd...

Clarification of influencing factors (cultivar planted, cultivation management, climatic conditions) affecting yields of summer maize (Zea mays L.) would provide valuable information for increasing yields further under variable climatic conditions. Here, we report actual maize yields in the Huanghuaihai region over the past 50 years (1957-2007), simulated yields of major varieties in different ...

One of the fundamental difficulties in Iranian rangelands is the existence of wide area of poor condition rangelands that their grazing capability is low due to continuous livestock grazing pressure. Astragalus flexilipes is one of the palatable plant species of rangelands in Iran, while its distribution is being decreased due to improper grazing. The aim of this study was to ...