We developed a simple, less laborious method to cultivate and isolate obligate anaerobic microorganisms using a six-well plate together with the AnaeroPack System, designated as the six-well plate method. The cultivation efficiency of this method, based on colony-forming units, colony formation time, and colony size, was evaluated with four authentic obligate anaerobes (two methanogenic archaea...

the main objective of this research was the measurement of soil characteristics, land evaluation and determination of potential yield of sugar beet in yekanat plain of marand. for doing this, firstly, yield potential of sugar beet was estimated by fao method and secondly land evaluation of different soil units for sugar beet cultivation was determined by parametric method. results showed that m...

Sponges host complex microbial communities of recognized ecological and biotechnological importance. Extensive cultivation efforts have been made to isolate sponge bacteria, but most still elude cultivation. To identify the bottlenecks of sponge bacterial cultivation, we combined high-throughput 16S rRNA gene sequencing with a variety of cultivation media and incubation conditions. We aimed to ...

BACKGROUND The natural ratios of carbon (C), nitrogen (N), and sulfur (S) stable isotopes can be varied in some specific living organisms owing to various isotopic fractionation processes in nature. Therefore, the analysis of C, N, and S stable isotope ratios in ginseng can provide a feasible method for determining ginseng authenticity depending on the cultivation land and type of fertilizer. ...

BACKGROUND Here we describe a novel cultivation method, called EnBasetrade mark, or enzyme-based-substrate-delivery, for the growth of microorganisms in millilitre and sub-millilitre scale which yields 5 to 20 times higher cell densities compared to standard methods. The novel method can be directly applied in microwell plates and shake flasks without any requirements for additional sensors or ...

Fibroblast cell cultivation method was evaluated by using natural polymers sourced from pulp cellulose in their hydrogel forms. The pulp sourced was offered as an alternative for the preparation of hydrogel films when the cellulose was dissolved in dimethylacetamide/lithium choride (DMAc/LiCl) solution and converted hydrogels having flexible and transparent properties. The cultivation of the fi...

Entamoeba histolytica, the causative agent for human amoebiasis, is among the most deadly parasites, accounting for the second highest mortality rate among parasitic diseases. Because this parasite dwells in low oxygen tension, for its cultivation, microaerophilic conditions are required to mimick the human gut environment. Several methods developed for optimal growth environment are commercial...

Preventive measures against soil-borne diseases need to be implemented before cultivation because very few countermeasures are available after the development of diseases. Some soils suppress soil-borne diseases despite the presence of a high population density of pathogens. If the suppressiveness of soil against soil-borne diseases may be predicted and diagnosed for crop fields, it may be poss...

studying of climatic elements  are one of the most important factors   influencing on agricultural crops  production and developing of agriculture activities in every region that led to increasing production . by studying of agro climatology  can be determined potential facilities in different areas  and used optimum of  facilities . this research was established  by determining  temperature po...