There is a variety of methods used for growing plants indoor for laboratory research. In most cases plant research requires germination and growth of plants. Often, people have adapted plant cultivation protocols to the conditions and materials at hand in their own laboratory and growth facilities. Here I will provide a guide for growing some of the most frequently used plant species for resear...

A REPORT by a working party of the Public Health Laboratory Service on the laboratory diagnosis of tuberculosis, reported in the Monthly Bulletin of the Ministry of Health and Public Health Laboratory Service (August, 1952, Vol. 11, p. 187 onwards) and referred to in the Bulletin of Hygiene (September, 1953, 28, 748), lays out the best methods of homogenization of sputa prior to culture on Lowe...

Cultivation of both human and non-human species of Plasmodium spp., the causal agent of malaria, has been a major research success, leading to a greater understanding of the parasite. Efforts at cultivating the organisms in vitro are complicated by the parasites' alternating between a human host and an arthropod vector, each having its own set of physiological, metabolic, and nutritional parame...

Implantation support medium transfer (ISME-T) is referred as cultivation and transfer of embryos using advanced enriched medium that all nutrients and energy sources needed for an optimal embryo development. They may serve as cultivation as well as transferring medium. Enriched cultivation medium reduces the embryo stress through in vitro fertilization (IVF) process by providing a more physiolo...

abstract agriculture activity is obvious type of unsafely and risky activities that this part had dealt with lack of trast about amount of production and price as unpleasant atmosphere phenomona, calamities, kind of plant diseases and change in prices. insurance is one of important cases for decreasing risk in agriculture. the main of this research is consideration crop insurance’s affect on op...

Pseudorabies virus has been cultivated in series in rabbit testicle, guinea pig testicle, and chick embryo media, and its growth requirements have been studied. Intranuclear inclusions, similar to those produced by pseudorabies virus in vivo, have been found in rabbit testicle cultures. The virus has not changed its pathogenic properties for rabbits, guinea pigs, or mice during the course of cu...

The authors have developed a method for large-scale isolation of metaphase chromosomes from HeLa cells. The distinguishing feature of this method is the use of a pH sufficiently low (about 3) to stabilize the chromosomes against mechanical damage. Many milligrams of fairly pure, morphologically intact chromosomes can be isolated in 8 hr or less of total working time. The isolated chromosomes co...

1. Spirochoeta gallinarum can be cultivated in suitable artificial media for many successive generations and probably for indefinite periods. The presence of fresh tissue and a certain amount of oxygen seems to be essential for its growth. No perceptible odor is produced in the cultures. 2. The maximum growth of Spirochoeta gallinarum is reached on about the fifth day, but the phase of degenera...