An Aedes aegypti-specific, fluorogenic probe hydrolysis (Taq-Man), polymerase chain reaction assay was developed for real-time screening using a field-deployable thermocycler. Laboratory-based testing of A. aegypti, A. aegypti (Trinidad strain), Culex pipiens, Culex quinquefasciatus, Anopheles stephensi, and Ochlerotatus taeniorhynchus individual adult mosquitoes and mixed pools (n = 10) demons...

The mosquitocidal glycoprotein endotoxin of Bacillus thuringiensis subsp. israelensis was digested with chymotrypsin to yield protease-resistant domains which were then separated from smaller protease digestion products by high-performance liquid chromatography. Once purified, the domains no longer bound wheat germ agglutinin, a lectin which binds N-acetylglucosamine (GlcNAc) and GlcNAc oligome...

Bioassay tests were carried out to investigate the susceptibility status of two (3rd and 4th) instar larvae of Culex quenquifasciatus Say populations of Dhaka city against two organophosphate (malathion and chloropyrifos) and two pyrithroid (cypermethrin and lambdacyhalothrin) insecticides. The LC50 values of malathion, chloropyrifos, cypermetrin and lambdacyhalothrin against the 3rd instar lar...

Living fossils” refers to plants that are similar to extinct ones. One classic example –Cycads, prominent in the Jurassic and Cretaceous period but recently the cycads have started to disappear. Cycas circinalis L. commonly known as Queen Sago is an endemic restricted to the Western Ghats, was investigated for larvicidal activity. The fourth instar larvae of Aedes aegypti and Culex quinquefasci...

The histochemical effects of the lethal concentration that kills 50% of larvae (LC50) of three biological agents, abamectin, Bacillus thuringiensis and spinosad on the carbohydrates (polysaccharides), proteins, nucleic acids and lipids content of the midgut and fat bodies of Culex pipiens 2nd instar larvae were studied. The results showed that the three tested compounds reduced the carbohydrate...

1School of Public Health and Institute of Health Research, Tehran University of Medical Science, Tehran, Islamic Republic of Iran. Received: 22/10/02; accepted: 18/08/03 ABSTRACT An insecticide containing azadirachtin, a neem tree (Azadirachta indica) extract, was tested against mosquito larvae in the Islamic Republic of Iran under laboratory and field conditions. LC50 and LC90 values for Neema...

Lysinibacillus sphaericus OT4b.25 is a native Colombian strain isolated from coleopteran larvae in an oak forest near Bogotá D.C.; this strain has shown high levels of pathogenic activity against Culex quinquefasciatus larvae in laboratory assays compared to that of other members of the same species. Using Pacific Biosciences sequencing technology, we propose a chromosomal contig of 4,665,775 b...

Many invasive species succeed in becoming established in new locations because of their competitive superiority to native species. This has been shown in several examples involving mosquitoes. In this study, we examined the interspecific competition between mosquito larvae of a well-established, non-native species, Culex pipiens, and those of its ecologically similar, native congener Culex rest...

Objective: The orange-red spectrum of  the skin of  electric yellow (Labidochromis caeruleus) is  one  of  the  idealistic and  necessary  schemas sought  by  aquarists  and commercial producers. In this study, the effect of live foods meal on the skin coloration of juvenile electric yellow was examined. Methods: 270 fish with an average living body weight was 0.42 ± 0.11 g, and averag...