UNLABELLED Protein crosslinking has been used for decades to derive structural information about proteins and protein complexes. Only recently, however, it became possible to map the amino acids involved in the crosslinks with the advent of high resolution mass spectrometry (MS). Here, we present Crossfinder, which automates the search for crosslinks formed by site-specifically incorporated cro...

The crosslinks of carbon nanotubes (CNT) film has been demonstrated to owing the ability reinforce quasi-static mechanical properties. But it is unclear whether improve ballistic impact resistance CNT film. Here, we investigated with by combining micro-ballistic experiments coarse-grained molecular dynamics (CGMD) simulations. quantitatively characterized in terms specific penetration energy. M...

When chromatin is photoreacted with psoralen, crosslinks occur preferentially in the linker DNA between nucleosomes. The pattern of these crosslinks can be analysed by exonuclease digestion of random DNA fragments, since the exonucleases tested stop at sites of psoralen-crosslinks. Further digestion of these fragments with Sl-nuclease leads to DNA fragments of nucleosomal and polynucleosomal si...

Introduction. Morphological and biomechanical features of the thoracolumbar junction determine large number cases traumatic bone injuries. Reconstructive stabilizing surgeries performed in this area, due to significant load on both elements hardware bony structures, require high reliability fixation. Objective. To study stress-strain state model section spine after Th12-L1 vertebrae resection w...

Front Cover: In article number 2300014, Yoshitake Suganuma and James A. Elliott investigate the effect of crosslink density on mechanical properties isotactic polypropylene (iPP) by dissipative particle dynamics (DPD) method. Coarse-grained structures iPP (blue chains) with different numbers crosslinks (magenta spheres) are developed from an all-atom model Bayesian optimization, their tensile t...

Although many anticancer drugs exert their biological activity by forming DNA interstrand crosslinks (ICLs), the thermodynamics of biologically relevant long crosslinked DNAs has not been intensively studied in contrast to short duplexes. Here, we carry out computer modeling of the shift of melting temperature of long DNAs caused by ICLs taking into account crosslinking effect in itself and con...