Blood metabolic profiles were determined from 40 days prepartum to 60 days postpartum in Uruguayan Holstein (UH) (n = 13) and UH x New Zealand Holstein-Friesian first cross (UH-NZHF) (n = 13) cows with seven in each group experiencing their second lactation (L2) and six experiencing their third lactation (L3). Non-esterified fatty acids increased around calving and tended to be greater in UH-L3...

The objective was to determine whether transfer of fresh or vitrified embryos produced in vitro with sex-sorted semen improves pregnancy and calving rates during summer in lactating dairy cows compared with artificial insemination (AI). Lactating dairy cows (n=722) were enrolled during summer months at 2 commercial dairies in Central Texas and randomly assigned to 1 of 3 treatments: AI with con...

The effect of dietary energy balance (EB) on growth of ovarian follicles was tested. Cows (n = 9) were fed a high energy diet (HE diet; positive EB; n = 4) or switched to a low energy diet (LE diet; negative EB; n = 5) during the preovulatory period. Non-esterified fatty acids (NEFA) were greater in cows fed the LE diet. Concentrations of luteinizing hormone (LH) were similar in HE and LE cows....

We wished to determine the expression of trafficking/adhesion molecules on the surface of lymphocytes isolated from infected mammary glands of cows challenged with either Serratia marcescens or Staphylococcus uberis. Healthy Holstein cows in mid lactation were infected by intramammary infusion with S. marcescens or S. uberis. Following infection, milk samples were collected at various time poin...

OBJECTIVE To evaluate rumen transfaunation after surgical correction of left-sided displacement of the abomasum (LDA) in cows. DESIGN Prospective clinical trial. ANIMALS 20 multiparous cows with LDA. PROCEDURES Cows with LDA were treated surgically (day 0). On days 0 (immediately after surgery) and 1, 10 cows each received 10 L of rumen fluid (transfaunated group) or 10 L of water (contro...

The objectives were to examine the effects of bovine somatotropin (bST), pregnancy, and dietary fatty acids on expression of key endometrial genes and proteins regulating prostaglandin synthesis in lactating dairy cows. Two diets were fed, at about 17 d in milk (DIM), in which oil of whole cottonseed (control diet) was compared with calcium salts of fish oil-enriched lipid (FO). Ovulation was s...

Data of 42 balance measurements from dry and lactating Holstein cows and blood and urine samples from 24 Japanese Black cows were collected to evaluate the potential for predicting urinary nitrogen (N) excretion from plasma urea nitrogen (PUN). Similar positive correlations were obtained between N intake and apparent N absorption in dry and lactating cows. The regression equations of N intake o...

Cow size, reproductive traits and calf performance through weaning were evaluated in a range environment for Simmental (S) x Hereford (H) and Angus (A) x H crosses in two-breed rotations and straightbred H. Data were grouped into seven dam breed categories: straightbred Hereford (H), crossbred F1 S x H cows (SH), S x H cows of low percentage H (SHS), S x H cows of high percentage H (HSH), F1 A ...

Our objective was to determine the effect of level and chemical form of dietary selenium on productivity of beef cows, concentrations of triiodothyronine (T3), and thyroxine (T4) in plasma, and immunoglobulins (IgG and IgM) in plasma and colostrum of cows. Pregnant cows (n = 60) were randomly allocated among four dietary treatments of 20, 60, or 120 ppm Se as selenite and 60 ppm as selenomethio...

The objective of this study was to investigate if cows milked twice per day have more difficulty lying down and getting up and spend less time lying than cows milked three times per day. Seventeen cows of the Swedish Red and White Cattle Breed were studied, seven of which were milked twice daily (2M) and ten were milked three times (3M) daily. They were kept in individual cubicles, that were cl...