Revolution in plant biotechnology can be categorized into in vitro culture and genetic transformation. Plant regeneration was successfully achieved in 1950’s while production of transgenic plant was accomplished in 80’s. For production of transformants, in vitro culturing strategies are prerequisite. Soybean has been cultured through organogenesis and embryogenesis but still it is considered re...

The present study aimed at establishing a complete plant regeneration protocol for Didymopanax morototoni (matchwood), a native Brazilian forest species. Four types of explants (root, shoot, node, and cotyledonary leaves) were obtained from in vitro germinated seeds. In the first step, woody plant medium (WPM) with casein hydrolysate (250 mgL-1 ) and 2,4-D (1.0 and 5.0 mgL-1) were used combined...

To accelerate breeding of Pinus massoniana Lamb. resistance to pine wilt disease (PWD), caused by the pinewood nematode (PWN), Bursaphelenchus xylophilus, a protocol was established for in vitro propagation P. and evaluation regenerated microshoots from different clones PWN. Axillary bud induction achieved culturing cotyledonary node explants 3-week-old seedlings Gupta Durzan (DCR) medium that ...

Cerastium candidissimum Correns is an endangered species. Nevertheless, its ornamental characteristics make it ideal candidate for commercial use as a new and landscape plant. As there are no references to the in vitro propagation of this species, present research aims comparatively investigate potential that may have three different populations plant, from Mounts Parnassos (PS), Parnitha (PR),...

Some large-seeded tree species have cotyledonary reserves that persist for months after seedling establishment. We carried out two screened growing-house experiments with seedlings of Gustavia superba (Lecythidaceae) to test hypotheses proposed to explain why cotyledons are retained. We grew seedlings from large and small seeds in sun and shade to determine if cotyledon reserves supplement phot...

An efficient protocol for plant regeneration has been developed for crop improvement programs. The present study was designed to assess the regeneration response of cotyledonary node explant taken from the germinating seeds of peanut (Arachis hypogaea L.). Complete plants were regenerated from in vitro cultured sectioned cotyledonary nodes. Multiple shoots arose on 6-benzylaminopurine (BAP) sup...

Common beans (Phaseolus vulgaris L.), like many legumes, are rich in iron, zinc, and certain other microelements that are generally found to be in low concentrations in cereals, other seed crops, and root or tubers and therefore are good candidates for biofortification. But a quandary exists in common bean biofortification: namely that the distribution of iron has been found to be variable betw...

Efficient in vitro plantlet regeneration is an important step to successfully transform genes for the improvement of agronomic traits. A combination 6-benzylaminopurine (BAP) and thidiazuron (TDZ) plant growth regulators was applied evaluate shoot capacity whereas α-naphthalene acetic acid (NAA) with (BAP), 2, 4-dichlorophenoxyacetic (2, 4-D) were tested optimize root induction two peanut culti...

Embryo rescue refers to an in vitro technology have been used for propagating plants under from poorly developed embryos by avoiding embryo abortion. The aim of the present study was regeneration a medicinally wild tree species Buchanania lanzan through method. Isolated immature dissected out de-coded seeds collected 15 years old trees were cultured Woody Plant Medium (WPM) test germination. 10...