The present work was designed to study the effect of different concentrations of bovine serum albumin (BSA) in extender on post-thaw quality of buffalo spermatozoa. Semen was collected from three buffalo bulls with artificial vagina (42°C) for three weeks (replicates). Qualifying semen ejaculate from each bull was split into five aliquots and diluted in tris-citric egg yolk extender containing ...

Caffeine is a well-known sperm motility stimulator, however, its effects on cooling-stored ram semen are unknown. The aim of the study was to examine the effect of caffeine on selected motility and viability indices of cooling-stored ram spermatozoa. Sperm ejaculates from 4 rams were diluted (1:3) in a Triladyl extender. Samples were stored for 96 h at 4-5 °C in two sets. In the first set used ...

The mobility of pooled turkey sperm following various storage regimens was assessed by objectively measuring the ability of sperm to penetrate a 2% Accudenz [5-(N-2,3-dihydroxypropylacetamido)-2,4,6-tri-iodo-N,N'-bis(2,3-dihydroxypropyl)isophthalamide] solution at 41 C. When semen was diluted with Beltsville poultry semen extender and stored at 5 C with agitation at 150 rpm, sperm mobility decl...

air cooled blast furnace slag is a by product made of gradually air cooled molten blast furnace slag, and is often stored in stockpiles near the iron mills and usually occupy a wide area around the iron mills. from this perspective the further use of these materials can have its own merits. air cooled blast furnace slag can be used in asphalt mixtures because of their proper frictional properti...

OBJECTIVE To evaluate the effectiveness of liquid nitrogen (LN(2)) vapor (indirect contact method) for the storage of human semen. DESIGN Experimental study. SETTING University hospital-based fertility center. PATIENT(S) We evaluated 150 patients with normal sperm parameters. INTERVENTION(S) Human semen (N = 120) was mixed with Semen Freezing Medium, divided into two groups, frozen, and...

The detection capabilities of both laser and high-intensity quartz arc tubes were evaluated. The Spectra-Physics Model 171-19, 18-W argon ion laser and Laser Sonics Model CS-2, 200-mW air-cooled argon ion laser were compared with Payton Scientific's Luma-Print, high-intensity quartz arc tube. The light sources were evaluated as to their detection limits for various biological stains. The stains...

Considerable scope exists for the use of AI in camel breeding programmes but the technology is not fully developed. The fertility of both fresh and liquid-stored (24 h; 4°C) dromedary camel semen diluted in either Green Buffer (GB) or INRA-96 (INRA) was assessed. Different insemination doses were investigated: 150 and 300 x 10 motile sperm for fresh sperm, and 150, 300 and 600 x 10 motile sperm...

the aim of the present study was to investigate the effect of in vitro supplementation of selenium on fresh and frozen spermatozoa quality of buffalo (bubalus bubalis) bulls. five healthy buffalo bulls (5 ejaculates from each bull) were used. each ejaculate was diluted at 37 ˚c with tris-based extender containing 0 (control), 0.5, 1, 2, 4 and 8 μg ml-1 sodium selenite and the sperm motility and...