miRNAs as one of the potential therapeutic agents have been recently considered for cancer treatment. AS1411 (aptNCL) is a DNA aptamer specifically binding to nucleolin protein on the cancer cell surface with antiproliferative effect. The aim of the study was to develop a conjugate consisted of aptNCL (as targeted delivery of therapeutic agent) and miRNA let-7d (as a tumor suppressor) using two...

The Laplacian energy of a graph sums up the absolute values of the differences of average degree and eigenvalues of the Laplace matrix of the graph. This spectral graph parameter is upper bounded by the energy obtained when replacing the eigenvalues with the conjugate degree sequence of the graph, in which the i-th number counts the nodes having degree at least i. Because the sequences of eigen...

The HIV proviral genome contains two copies of a 16 bp homopurine.homopyrimidine sequence which overlaps the recognition and cleavage site of the Dra I restriction enzyme. Psoralen was attached to the 16-mer homopyrimidine oligonucleotide, d5'(TTTTCT-TTTCCCCCCT)3', which forms a triple helix with this HIV proviral sequence. Two plasmids, containing part of the HIV proviral DNA, with either one ...

In this paper we study ultraflat sequences (Pn) of unimodular polynomials Pn ∈ Kn in general, not necessarily those produced by Kahane in his paper [Ka]. We examine how far is a sequence (Pn) of unimodular polynomials Pn ∈ Kn from being conjugate reciprocal. Our main results include the following. Theorem. Given a sequence (εn) of positive numbers tending to 0, assume that (Pn) is a (εn)-ultraf...

We developed an improved microtiter-based assay for the detection of polymerase chain reaction (PCR)-amplified DNA sequences. The synthetic DNA sequences used to prime the PCR were labeled with biotin at their 5' ends so that the specific PCR product was labeled with biotin. Following amplification, an aliquot of the PCR product was denatured and hybridized to a capture DNA sequence immobilized...

We establish some identities relating two sequences that are, as explained, related to the Tribonacci sequence. One of these sequences bears the same resemblance to the Tribonacci sequence as the Lucas sequence does to the Fibonacci sequence. Defining a matrix that we call Tribomatrix, which extends the Fibonacci matrix, we see that the other sequence is related to the sum of the determinants o...

Grone and Merris [5] conjectured that the Laplacian spectrum of a graph is majorized by its conjugate vertex degree sequence. In this paper, we prove that this conjecture holds for a class of graphs including trees. We also show that this conjecture and its generalization to graphs with Dirichlet boundary conditions are equivalent.

An efficient procedure for preparing enantiopure polycyclic ethers is reported. The protocol is based on the photo-oxidation/conjugate addition sequence over a chiral functionalized furan, which was prepared from commercially available tri-O-acetyl-D-glucal. The Michael addition step afforded two products with the same absolute configuration from a mixture of diastereomers.

We prove that the symbolic dynamical system generated by a purely substitutive Arnoux-Rauzy sequence is measurably conjugate to a toral translation. The proof is based on an explicit construction of a fundamental domain with fractal boundary (a Rauzy fractal) for this toral translation. Communicated by Pierre Liardet Dedicated to the memory of Gérard Rauzy