The bacterial diversity associated with biofilm-forming ability was studied. Eighteen bacterial strains were isolated from a microbial film collected from the roof of an old house located in Sfax, Tunisia. The purity of these microorganisms was confirmed by microscopic observation after repeated streaking on a Tryptic Soy agar medium. Biofilm formation was estimated using preliminary tests incl...

A preliminary study to characterize filamentous bacteria, whose presence is related to high mortality of Litopenaeus vannamei larvae cultured in Santa Catarina State, Brazil, is reported. The extract of infected larvae was diluted in different concentrations, cultured in marine agar (Difco, Marine Agar 2216) and incubated at 30 degrees C for 48 hours. The biochemical characterization included h...

The objectives of the present study were isolation, identification and characterization of xylanase producing fungi, optimization of medium composition and cultural conditions for xylanase enzyme production using cheaper sources. The fungal strains were isolated from garden soil by serial dilution technique and Aspergillus niger was identified and isolated in pure form. In conformation screenin...

Chorion is the major component of silkmoth eggshell. More than 95% of its dry mass consists of the A and B families of low molecular weight structural proteins, which have remarkable mechanical and chemical properties protecting the oocyte and developing embryo from environmental hazards. We present data from FT-Raman spectroscopy of silkmoth chorion and amyloid-like fibrils formed from peptide...

This research aim to preliminarily investigate biofilm production among Vibrio species isolated from Siloso beach, Singapore. 100 μL of sea water sample collected was plated on Congo Red Agar (CRA) using spread plate method. The plates were incubated at 30°C for 24 hours. Forty – six biofilm producing black colonies with dry crystalline consistence were further transferred aseptically into fres...

background and objectives: discharge of wastewater from textile dyeing industries has been a problem in terms of pollution and treatment of these waters is a great task. keeping this in mind, the aim of our current research is to study the effect of various bioprocess variables on decolorization of an azo dye, congo red, by a fungal isolate, aspergillus niger hm11. materials and methods: centra...

carbon activated powdered was prepared from activated carbon derived from olive stone. then byusing the surface methodology, many parameters such as ph, furnace temperature (t), acid ratio,time of activation that affect on the qualities prepared activated carbon produced and its efficiencywere investigated. in addition, the adsorption of congo red onto the activated olive stone carbonactive was...

Coagulase negative staphylococci (CoNS) have become a major public health problem due to an increase in invasive procedures and antibiotic resistance. The prevalence and some virulence factors of CoNS species isolated from clinical samples obtained from a hospital in Benin-City, Nigeria was investigated. A total of 79 clinical samples were collected from clinical specimens over a six month peri...

Congo red (CR) binding, monitored by characteristic yellow-green birefringence under crossed polarization has been used as a diagnostic test for the presence of amyloid in tissue sections for several decades. This assay is also widely used for the characterization of in vitro amyloid fibrils. In order to probe the structural specificity of Congo red binding to amyloid fibrils we have used an in...

Congo red has been used to identify amyloid fibrils in tissues for more than 80 years and is also a weak inhibitor to both amyloid-beta fibril formation and toxicity. However, the specificity of the binding and its inhibition mechanism remain unclear. Using all-atom molecular dynamics simulations with the explicit solvent model, we have identified and characterized two specific binding modes of...