Several commercial radioimmunoassay kits are now marketed specifically for determination of thyrotropin (TSH) from whole-blood specimens collected on filter paper in neonatal screening programs. We have compared five kits in use in such screening programs in the United States. The reagent kits from Becton Dickinson, Neometrics, and Nuclear Medical Systems gave similar satisfactory results. That...

DNA purification is a routine procedure in most plant laboratories. Although different kits are available in the market allowing convenient DNA purification, the cumulative cost of purchasing multiple kits for a laboratory can be staggering. Here, we describe a protocol using homemade silicon dioxide matrix for DNA purification from Escherichia coli and Agrobacterium tumefaciens cells, PCR and ...

Experiments were performed to evaluate five commercial kit assays used for the detection of antibodies to dsDNA. The kits were compared using a performance index score as recommended by the guidelines of the European Committee for Clinical Laboratory Standards. The highest performance score was obtained using the radioimmunoassay from Immunodiagnostic Services Ltd, with the Amersham kit second,...

The “in house” PCR method and four commercial PCR kits (designed symbolically as A, B, C, and D) for the detection of Mycoplasma synoviae (MS) in tracheal swabs of infected experimentally SPF chickens were compared. The chickens were inoculated intranasally with 1x10 colour change unit (cfu)/mL of ATCC reference MS strain, and at 4, 7, 14, 28, and 35 d post infection (d.p.i.), the tracheal samp...