Chromatography is an important biophysical technique that enables the separation, identification, and purification of the components of a mixture for qualitative and quantitative analysis. Proteins can be purified based on characteristics such as size and shape, total charge, hydrophobic groups present on the surface, and binding capacity with the stationary phase. Four separation techniques ba...

Earlier column chromatography was used for preparative purposes as well reaction control in organic synthesis. Column is an extremely tedious stage any laboratory and can quickly become a point of congestion process lab. Flash technique developed modification chromatography. This air pressure driven comprising medium short chromatography, optimised rapid separation compounds. Modern flash chrom...