The study was conducted to examine the expression of collagen type I and II in the different cartilage layers in relation to other ECM molecules during the progression of early osteoarthritic degeneration in human articular cartilage (AC). Quantitative real-time (RT)-PCR and colorimetrical techniques were used for calibration of Photoshop-based image analysis in detecting such lesions. Immuno...

introduction & objective: the brain choroids plexus (bcp) plays an important role in the cerebrospinal fluid (csf) production, but its characterization is still incomplete. collagen type iv, is one of the most important proteins of basement membrane (bm) and extracellular matrix (ecm) of bcp. in the present study we investigated the differential period of type iv collagen in basement membrane o...

background: nicotine can pass through placental blood barrier and accumulate in the developing organs of fetus. also, entering the breast milk, nicotine can have an effect on the neonates. investigations have showed that collagen iv is one of the most important micro vessels basement membrane components. objective:  in this study, the effect of maternal nicotine exposure in pre and postnatal pe...

We have found that intradermal injection of native type II collagen extracted from human, chick or rat cartilage induces an inflammatory arthritis in approximately 40% of rats of several strains whether complete Freund's adjuvant or incomplete Freund's adjuvant is used. Type I or III collagen extracted from skin, cartilage proteoglycans and alpha1(II) chains were incapable of eliciting arthriti...

In avian hypertrophic cartilage, the maturation pathway from mesenchymal prechondrogenic cells to fully differentiated hypertrophic chondrocytes proceeds through differentiation stages that can be defined by biochemical markers and several other parameters such as cell volume, cellular phenotype and growth kinetics. The chondrocyte differentiation process is divided into three schematic cellula...

BACKGROUND Mild developmental dysplasia of hip (DDH) causes high morbidity of osteoarthritis (OA) on adult. It is thought that change of collagen and proteoglycans in cartilage may be the direct reasons for osteoarthritis. OBJECTIVE To detect the changes of the expressions of type II collagen of acetabular cartilage in early DDH and to investigate the relevance between type II collagen and th...

PURPOSE To elucidate the function of type II collagen in the development and diseases of the eye by analyzing the temporospatial expression of the long (IIA) and short (IIB) isoforms of type II collagen in the normal and transgenic Dell mice. METHODS Normal and Dell transgenic embryos harboring a deletion mutation in the pro alpha 1 (II) collagen chain were studied from day 10.5 of embryonic ...

Achondrogenesis II-hypochondrogenesis and severe spondyloepiphyseal dysplasia congenita (SEDC) are lethal forms of dwarfism caused by dominant mutations in the type II collagen gene (COL2A1). To identify the underlying defect in seven cases with this group of conditions, we used the combined strategy of cartilage protein analysis and COL2A1 mutation analysis. Overmodified type II collagen and t...

PURPOSE To determine whether aging vitreous collagen fibrils undergo ultrastructural changes that might underlie vitreous liquefaction and posterior vitreous detachment. METHODS Vitreous collagen fibrils from 21 human subjects (age range, 3-89 years) and from bovine eyes were isolated on electron microscopy grids. Cupromeronic blue labeling in the presence of 0.3 M MgCl(2) and immunogold labe...

We sought to determine the cartilage repair capacity of WIN-34B in the collagenase-induced osteoarthritis rabbit model and in progenitor cells from subchondral bone. The cartilage protective effect of WIN-34B was measured by clinical and histological scores, cartilage area, and proteoglycan and collagen contents in the collagenase-induced osteoarthritis rabbit model. The efficacy of chondrogeni...