The knee meniscus contains a mixed population of cells that exhibit fibroblastic as well as chondrocytic characteristics. Tissue engineering studies and future therapies for the meniscus require a large population of cells that are seeded on scaffolds. To achieve this, monolayer expansion is often used as a technique to increase cell number. However, the phenotype of these cells may be signific...

The treatment of large-area bone defects remains a challenge; however, various strategies 20 have been developed to improve the performances of scaffolds in bone tissue engineering. In this 21 study, poly(lactide-co-glycolide)/hydroxyapatite (PLGA/HA) scaffold was coated with 22 Asp-Gly-Glu-Ala (DGEA)-incorporated collagen for the repair of rat skull defect. Our results 23 indicated that the me...

The aim of this work was to investigate the effect of silica coating on bioactivity and biodegradability of hydroxyapatite. In this purpose, we firstly attempted to synthesis hydroxyapatite (HA) nanoparticles and its silica coated (Si-HA) sample in collagen matrix using calcium chloride, sodium phosphate and sodium silicate. Characterization of the sample was carried out using Fourier transform...

Cell behavior in the presence of nanomaterials is typically explored through simple viability assays, but there is mounting evidence that nanomaterials can have more subtle effects on a variety of cellular functions. Previously our lab demonstrated that gold nanorods functionalized with polyelectrolyte multi-layers inhibited rat cardiac fibroblast-mediated remodeling of type I collagen scaffold...

The treatment of large-area bone defects remains a challenge; however, various strategies have been developed to improve the performances of scaffolds in bone tissue engineering. In this study, poly(lactide-co-glycolide)/hydroxyapatite (PLGA/HA) scaffold was coated with Asp-Gly-Glu-Ala (DGEA)-incorporated collagen for the repair of rat skull defect. Our results indicated that the mechanical str...

This study aims at evaluating the biocompatibility of titanium surfaces modified according two different ways: (i) deposition of a bio-inert, thin film of rutile TiO(2) by chemical vapour deposition (MOCVD), and (ii) biochemical treatment with collagen gel, in order to obtain a bio-interactive coating. Behind the comparison is the idea that either the bio-inert or the bio-active coating has spe...

The Ligament Advanced Reinforcement System has recently been widely used as the primary graft of choice in anterior cruciate ligament (ACL) reconstruction. But the biological graft-bone healing still remains a problem. Previous studies have shown that simvastatin (SIM) stimulates bone formation. The objective of this study was to investigate whether surface coating with collagen containing low-...

Cell structure depends on both matrix strain and stiffness, but their interactive effects are poorly understood. We investigated the interactive roles of matrix properties and stretching patterns on cell structure by uniaxially stretching U2OS cells expressing GFP-actin on silicone rubber sheets supporting either a surface-adsorbed coating or thick hydrogel of type-I collagen. Cells and their a...

In this study, we established that collagen gel, but not collagen gel coating, induced apoptosis exclusively in epithelial cell lines, which indicated that low substratum rigidity might trigger cell apoptosis. To confirm this, we used collagen gels with different rigidities due to cross-linking or physical disruption of collagen fibrils caused by sonication. We found that collagen gel-induced a...

Chitosan-collagen scaffolds were produced by freeze-drying method and coated with a thermoresponsive polymer poly(N, N-diethylacrylamide) (PDEAAm) to confer them temperature sensitivity using a non residue technology, the supercritical carbon dioxide (scCO2). All native and coated scaffolds were characterized by SEM, intrusion mercury porosimetry, swelling, biodegradability and mechanical analy...