1. Commercially available coenzyme A preparations were found to contain sufficient amounts of glutathione so that in the reaction with diketene significant quanties of acetoacetyl glutathione were formed. Unless allowances are made for this, significant errors in the interpretation of results can be made because liver mitochondria contain a very active acetoacetyl glutathione hydrolase. 2. Puri...

BACKGROUND Ischemia and subsequent reperfusion induce lipid peroxidation in the cerebrum of the fetal rat. The present study evaluated the antioxidant activity of endogenous coenzyme Q in protecting against the lipid peroxidation induced in the fetal rat brain by ischemia/reperfusion. METHODS We used wistar rats at day 19 of pregnancy. Fetal ischemia was induced by bilateral occlusion of the ...

The isolation of an adenylcobamide coenzyme that is required for the interconversion of glutamate and ,8-methylaspartate by a bacterial enzyme system has been described and some properties of the coenzyme have been reported (1, 2). The spectrum of the coenzyme is very different from that of the cobamide vitamins, such as cyanocobalamin (5,6-dimethylbenzimidazolylcobamide cyanide, vitamin Big) a...

Nanocapsule suspensions containing coenzyme Q10 were prepared by interfacial deposition. The nanocapsules showed characteristics compatible with dermal application: slightly acid pH, drug content close to 100%, particle size between 213 and 248 nm with low polydispersity and negative zeta potential. Three cosmetic formulations for skin application were developed, one with the free-coenzyme Q10,...

The potential protective effect of coenzyme Q10 against acute liver injury induced by a single dose of acetaminophen (700 mg/kg, p.o.) was investigated in rats. Coenzyme Q10 treatment was given as two i.p. injections, 10 mg/kg each, at 1 and 12 h following acetaminophen administration. Coenzyme Q10 significantly reduced the levels of serum aminotransferases, suppressed lipid peroxidation, preve...

Background: In-situ preservation of cauda epididymal spermatozoa at -10 degree centigrade with electrolyte free media for obtaining maximum functional gametes than preservation at 5 degree centigrade. Materials and Methods: Electrolyte free media prepared with soybean lecithin-glycerol, Coenzyme Q10 - glycerol and soybean lecithin - Coenzyme Q10- glycerol were inoculated separately into ligated...

When highly purified cobamide-dependent ribonucleotide reductase from Lacfobacillus leichmannii is incubated with synthetically prepared 5,6-dimethylbenzimidazolylcobamide 5’-deoxyadenosyl coenzyme containing tritium attached to carbon atom 5’ of the deoxyadenosyl moiety (DBCC-5’-3H), tritium is transferred from DBCC-5’-3H to Hz0 in a reaction requiring substrate, enzyme, and dithiol reductant....

Coenzyme Q(10), a lipid soluble benzoquinone, has excellent antioxidant and membrane stabilizing properties in the cardiac tissue. The present study quantitatively investigated the effects of coenzyme Q(10) on in vitro LDL oxidation. The formation of conjugated dienes, malondialdehyde (MDA) and electrophoretic mobility were monitored as markers of the oxidation of LDL, respectively. Coenzyme Q(...