The images obtained using a confocal laser scanning microscope (CLSM) alone cannot detect threedimensional (3D) structures, for example of the endothelial cells that compose blood vessels, and require segmentation for 3D interpretation. Here, we investigated the segmentation of hepatic sinusiodal veins, which form periodic 3D networks. We propose a new approach for image segmentation based on t...

The 3D pyramid compressor project at the University of Glasgow has developed a compressor for images obtained from CLSM device. The proposed method using a combination of image pyramid coder and vector quantization techniques has good performance at compressing confocal volume image data. An experiment was conducted on several kinds of CLSM data using the presented compressor compared to other ...

Additive manufacturing performed with solid particles can form microscopic structures based on the properties of the particles used. These microstructures will likely impact the performance of the product produced. Characterizing these microstructures is difficult since the interior of powders is a challenge to image. Most imaging techniques are limited to surface visualizations. We have establ...

Abstract The determination of a suitable correction for tip blunting is crucial in order to obtain useful mechanical properties from nanoindentation experiments. While typically the required area function acquired indentation reference material, direct imaging by methods an interesting alternative. In this paper, we investigate applicability confocal laser scanning microscopy (CLSM), atomic for...

Erosion of dentin results in a complex multi-layered lesion. Several methods have been used to measure erosive substance loss of dentin, but were found to have only limited agreement, in parts because they assess different structural parameters. The present study compared the agreement of four different methods (transversal microradiography [TMR], Confocal Laser Scanning Microscopy [CLSM], Lase...

In this work, alginate-chitosan microgel particles were formed at different pH levels with the aim of using them as viscoelastic interfacial layers, which confer emulsion stability to food systems. The particles’ size and structural characteristics determined laser diffraction, confocal microscopy (CLSM), time-domain nuclear magnetic resonance (TD-NMR). affected characteristics, larger lower le...

The aim of this work was to evaluate the performances recently developedin vivo imaging device Line-Field Confocal Optical Coherence Tomography (LC-OCT, DAMAE Medical) compared Laser Scanning Microscopy (CLSM, Vivascope 1500, Mavig) from a qualitative point view and assessment epidermal thickness according age. We conducted mono-centric open-label study. A total 17 subjects divided into three g...