The aim of the present study was to evaluate the effect of sperm reactive oxygen species (ROS) production and DNA changes on male fertility. For that purpose, six rams with significantly different pregnancy rates were used; these were classified as having high fertility, i.e. 59.4% average pregnancy rate, or low fertility, i.e. 23.1% average pregnancy rate. Sperm quality was assessed after a tw...

Strategies to increase the population and productivity of beef cattle can be implemented through reproductive management, such as artificial insemination (AI) using frozen semen. This study aims evaluate characteristics semen Limousin bulls different ages, which still used for programs up 12 years age. ages 3, 8, produced in 2021 at Artificial Insemination Center Singosari, East Java Province, ...

Cytogenetic studies were carried out on samples of Parapteronotus hasemani, Sternarchogiton preto and Sternarchorhamphus muelleri (Apteronotidae, Gymnotiformes) from the Amazon basin. The first two species exhibited both a 2n = 52 karyotype, but differed in their karyotypic formulae, distribution of constitutive heterochromatin, and chromosomal location of the NOR. The third species, Sternarcho...

Ten germplasms of three Crotalaria species viz. C. pallida (Acc. No. 4250, 4803, 4805, 4806, and 4807), incana 4790, 4801, 4804, 4809), juncea (Local) were investigated cytogenetically through Chromomycin A3 (CMA) 4′-6 diamidino-2-phenylindole (DAPI) fluorescent bandings. After CMA-banding, 10 showed 31 centromeric 34 terminal bands which indicated a tendency accumulating GC-rich sequences at r...

various cultured cells by actinomycin D or chromomycin A3 (1 1, 12, 23). In all these cell types, the nucleolar shape seems to reflect a low rate of nucleolar RNA synthesis. Although the ultrastructural morphology of undifferen tiated and well-differentiated cells of lymphosarcomas (poorly and well-differentiated malignant lymphomas) is very similar to leukemic lymphocytes in various stages of ...

The male karyotypes of Acmaeodera pilosellae persica Mannerheim, 1837 with 2n=20 (18+neoXY), Sphenoptera scovitzii Faldermann, 1835 (2n=38-46), Dicerca aenea validiuscula Semenov, 1895 - 2n=20 (18+Xyp) and Sphaerobothris aghababiani Volkovitsh et Kalashian, 1998 - 2n=16 (14+Xyp) were studied using conventional staining and different chromosome banding techniques: C-banding, AgNOR-banding, as we...

In vitro chemosensitivity of urological cancers was assessed by a human tumor colony forming assay (HTCA) and a 3H-thymidine incorporation assay. Primary tumor cells from 160 of 253 (63%) urological cancers showed adequate colony growth (greater than 30 colonies per well), and there was a 57% true positive and 100% true negative rate for predicting clinical response of anticancer agents. On the...