We have evaluated the performance of a centrifugal analyzer, specifically designed for hemostasis tests, for methods based on clotting time as measured by light scattering, or based on splitting of chromogenic substrates. The results were compared with those obtained with a semiautomated coagulometer (clotting tests) or with a manually operated photometer (chromogenic tests). In general, the re...

A 1-month old neonate urine sample yielded vanB Enterococcus faecium; nevertheless, the isolate alternatively showed susceptibility and resistance to vancomycin with bioMérieux Vitek2 (cards AST592, AST632, AST586), while glycopeptide resistance was detected by Liofilchem® vancomycin MIC Test Strip and disc along with the Chromatic VRE chromogenic medium. This communication emphasizes that, as ...

An amidolytic assay employing the chromogenic substrate S 2337 (Kabi Diagnostica) was used to assay factor X in 35 healthy controls and in 100 outpatients receiving oral anticoagulant therapy. This method correlated well with a coagulation assay of factor X in the control group (r = 0.88). When compared with two routine tests for the control of anticoagulant theray (Thrombotest and prothrombin ...

Imaging of reporter molecules such as fluorescent proteins in intact animals, tissues and cells has become an indispensable tool in cell biology. Imaging activity of enzymes, which is called metabolic mapping, provides information on subcellular localisation in combination with functions of the enzymes. The principle of metabolic mapping is imaging of the formation of a reaction product that is...

Novel 7-N-(beta-alanyl)aminophenoxazin-3-one salts 27a-d have been synthesized and tested as chromogenic substrates for beta-alanyl aminopeptidase, which is present in Pseudomonas aeruginosa, the most common respiratory pathogen in patients with cystic fibrosis. The biological results show that 7-N-(beta-alanyl)amino-1-pentylphenoxazin-3-one trifluoroacetate salt 27a is a chromogenic substrate ...

A group of five tests utilizing wheat germ and soybean lectins and chromogenic substrates (orthonitrophenyl-beta-D-galactopyranoside, gamma-glutamyl-beta-naphthylamide, and prolyl-beta-naphthylamide derivatives) was used as a rapid (30-min) method for the identification of Neisseria gonorrhoeae. The rapid method agreed with Minitek test results for all 126 N. gonorrhoeae isolates and all 39 non...