We compared and statistically evaluated the effectiveness of nine DNA extraction procedures by using frozen and dried samples of two silt loam soils and a silt loam wetland sediment with different organic matter contents. The effects of different chemical extractants (sodium dodecyl sulfate [SDS], chloroform, phenol, Chelex 100, and guanadinium isothiocyanate), different physical disruption met...

Goats’ milk adulteration with cows’ milk is becoming a big problem. In the past, the urea-polyacrylamide gel electrophoresis assay with different motility of αS1-casein has been applied for the identification of cows’ milk adulteration. The detection sensitivity is 1.0%. The aim of this study was to develop a faster and more sensitive method to detect cows’ milk which may be present in adultera...

Equine piroplasmosis is a tropical parasitic disease caused by several intraerythrocytic members of the genera Babesia and Theileria. These pathogens are transmitted multiple tick species considered as important exotic microorganisms in countries. causes significant economic losses due to abortions, decreased activity even death equines, making surveillance these infectious essential. In northe...

Whereas human red blood cells and protoplasts of leaves of Avena sativa show rotation only at discrete frequencies in the kHz-range of a linear alternating electric field, rotation of these cells is observed at practically every frequency in the MHz range (15 to 200 MHz). The cell rotation in the MHz-range can be explained in terms of field induced polarisation and orientation of permanent dipo...

Al toxicity in plants is related to the activity of Ae+ and AI­ hydroxy monomers in the soil solution, whereas Al complexed with ligands such as fluoride (F), s~lphate (SQ42-), and oxalate is not toxic. Estimation of toxic Al relies on measurement of "labile" Al after short contact times with colorimetric reagents or cation-exchange resins. However, shifts in equilibrium may result in non-toxic...

Our laboratory, like those of Daniels et al' and Melki et al,2 is conducting prenatal diagnoses of type 1 spinal muscular atrophy (SMA) by means of linkage analysis with 5ql3 polymorphisms. In our experience, a decision for pregnancy termination is taken if a diagnosis of SMA is made. Consequently, in the interests of generating a rapid prenatal SMA diagnosis, we have recently opted for a solel...