نتایج جستجو برای: chelex

تعداد نتایج: 261  

Journal: :The Journal of biological chemistry 1986
J P Reeves C A Bailey C C Hale

Na-Ca exchange activity in bovine cardiac sarcolemmal vesicles was stimulated up to 10-fold by preincubating the vesicles with 1 microM FeSO4 plus 1 mM dithiothreitol (DTT) in a NaCl medium. The increase in activity was not reversed upon removing the Fe and DTT. Stimulation of exchange activity under these conditions was completely blocked by 0.1 mM EDTA or o-phenanthroline; this suggests that ...

Journal: :Journal of medical genetics 1993
A MacKenzie A Besner N Roy

Our laboratory, like those of Daniels et al' and Melki et al,2 is conducting prenatal diagnoses of type 1 spinal muscular atrophy (SMA) by means of linkage analysis with 5ql3 polymorphisms. In our experience, a decision for pregnancy termination is taken if a diagnosis of SMA is made. Consequently, in the interests of generating a rapid prenatal SMA diagnosis, we have recently opted for a solel...

Journal: :Journal of environmental monitoring : JEM 2010
Martin M Shafer Dawn A Perkins Dagmara S Antkiewicz Elizabeth A Stone Tauseef A Quraishi James J Schauer

In this study a sensitive macrophage-based in vitro reactive oxygen species (ROS) assay was coupled with chemical fractionation tools and a year-long sampling program to further our understanding of the role of water-soluble metals in aerosol toxicity. The location is the polluted urban environment of Lahore, Pakistan, where we collected 24 h PM10 and PM2.5 samples every 6(th) day from January ...

2006
Yeong-Hsiang Cheng Su-Der Chen Ching-Feng Weng

Goats’ milk adulteration with cows’ milk is becoming a big problem. In the past, the urea-polyacrylamide gel electrophoresis assay with different motility of αS1-casein has been applied for the identification of cows’ milk adulteration. The detection sensitivity is 1.0%. The aim of this study was to develop a faster and more sensitive method to detect cows’ milk which may be present in adultera...

Journal: :Forensic Science International: Genetics Supplement Series 2019

Journal: :Methods in enzymology 2009
Helena Block Barbara Maertens Anne Spriestersbach Nicole Brinker Jan Kubicek Roland Fabis Jörg Labahn Frank Schäfer

This article reviews the development of immobilized-metal affinity chromatography (IMAC) and describes its most important applications. We provide an overview on the use of IMAC in protein fractionation and proteomics, in protein immobilization and detection, and on some special applications such as purification of immunoglobulins and the Chelex method. The most relevant application-purificatio...

2002
K. HOLOVSKÁ V. LENÁRTOVÁ P. JAVORSKÝ

Superoxide dismutase (SOD) isoenzymes of the rumen bacterium Streptococcus bovis 4/1 were studied. Native PAGE showed a single band of Mn-SOD, unaffected by 10 mM cyanide or 5 mM hydrogen peroxide under both aerobic and anaerobic growth conditions. When the metals were removed from the growth medium by Chelex 100, the addition of manganese increased enzymatic activity, while addition of iron in...

2011
Emmanuelle Uher Cécile Mirande-Bret Catherine Gourlay-Francé

A general comprehensive picture of kinetic methodologies in soil heavy metal mobilization research area ispresented in this paper. The author‟s experiences and the existing literature have been analysed and discussedbriefly in terms of merits and disadvantages of each kinetic approach in mobilty and bioavailability prediction.The contribution of three kinetic methodologies including...

Journal: :The Journal of General Physiology 1965
F. Norman Briggs Martin Fleishman

A high molecular weight fraction of a soluble Marsh muscle-relaxing preparation has been shown to contain a calcium-complexing substance. By examining the nature of the competition between this fraction and chelex-100 for calcium at various total calcium concentrations it has been possible to calculate the concentration and calcium stability constant of this calcium-complexing substance. Taking...

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