BACKGROUND The major cat allergen Fel d I consists of two polypeptide chains linked by disulfide bonds, each of which has been expressed in bacteria. To investigate the antigenic structure of Fel d I, antibody binding to the native molecule and to each recombinant chain were compared. METHODS Polyclonal human IgE and IgG antibodies and monoclonal antibodies (mAbs) to Fel d I were compared for...

In this study, the trajectories of four protomers corresponding to light chain tetramers human antibodies were obtained from MD simulations. The protomers, which should be symmetrically constructed, not completely symmetrical in DSSP analysis and 2D-RMSD showed that although secondary structure is approximately same, deconvolution part α helix leads asymmetry overall simulation. Although number...

A single-chain antibody construct was prepared containing the VH and VL regions of anti-(integrin alpha 4) antibody HP1/2, an interchain linker and a KDEL endoplasmic reticulum retention sequence. Intracellular expression of this single-chain antibody caused cell-surface expression of alpha 4 beta 1 integrin to be decreased by 80% on selected RD cells and by 65-100% on selected Jurkat cells, re...

Conditions were developed by which the separated H and L chains of gamma(2) globulins recombined to form four-chained molecules in good yields. In the absence of antigen, anti-2,4-dinitrophenyl (anti-DNP) H chains randomly reassociated with a mixture of antibody and non-specific gamma(2) globulin L chains. In the presence of a specific hapten, however, the antibody H chains preferentially inter...

Murine monoclonal antibody 2B8 specifically recognizes the CD20 phosphoprotein expressed on the surface of normal B lymphocytes and B-cell lymphomas. The light- and heavy-chain variable regions of 2B8 were cloned, after amplification by the polymerase chain reaction, into a cDNA expression vector that contained human IgG1 heavy chain and human kappa-light chain constant regions. High-level expr...