Sucrase-isomaltase (S-I) is an intestinal membrane enzyme consisting of two active moieties each with its hydrolytic site available for nutrient digestion at the luminal-cell interface. At least 90% of hydrolytic activity can be localized to the brush border membrane and the remainder in the cytoplasm has been considered to originate from brush border contamination. The intracellular cytosol fr...

Na+-glucose cotransporter (SGLT1) expression and the role of actin in epidermal growth factor (EGF)-induced alterations in glucose transport and brush-border surface area were examined in New Zealand White rabbit jejunal loops. In separate experiments, EGF or EGF concurrent with cytochalasin D, an inhibitor of actin polymerization, was administered to the experimental loop and compared with its...

The filamentous brush border glycocalyx forming the 'enteric surface coat' of the intestinal epithelium is composed in rabbits of a 400 kDa mucin-type glycoprotein, which was purified using the 3A4 monoclonal antibody. This monoclonal antibody recognizes a filamentous brush border glycocalyx-specific glycosidic structure containing an O-acetylated sialic acid, which is absent from all the other...

To determine the functional specificity of intestinal brush-border pteroylpolyglutamate hydrolase (PPH), we compared the regional location of in vivo hydrolysis of pteroyltriglutamate (PteGlu3) with the location of activity and immunoreactivity of the enzyme in the pig. After in vivo incubations, PteGlu3 hydrolytic products were recovered from intestinal segments in the jejunum but not from the...

BACKGROUND In qualitative diagnosis of bile duct stenosis, single diagnostic measure is difficult to make a correct diagnosis, to combine several diagnostic techniques may be helpful to make an accurate diagnosis. The aim of this study was to evaluate the value of intraductal ultrasonography (IDUS), endoscopic brush cytology and K-ras, P53 gene mutation in the early diagnosis of malignant bilia...

By use of a combined experimental and theoretical approach, a model poly(ethylene oxide) (PEO) brush system, prepared by spreading a poly(ethylene oxide)-poly(n-butyl acrylate) (PEO-PnBA) amphiphilic diblock copolymer onto an air-water interface, was investigated. The polymer segment density profiles of the PEO brush in the direction normal to the air-water interface under various grafting dens...

Polymers with advanced topological architectures are promising materials for wide applications due to their structure-generated unique properties different from that of the linear analogues. The elegant integration of stimuli-responsive polymers with such advanced architectures can create novel materials with virtues from both moieties, are thus a hot subject of research for both fundamental an...

The effect of total parenteral nutrition on nutrients absorption and glycoprotein changes of brush border membrane was examined in rat small intestine. In total parenteral nutrition rats, a marked decrease in activity of brush border enzymes was observed mainly in the proximal and middle segments of the intestine. Galactose perfusion of jejunal segment showed that hexose absorption was signific...

statement of the problem: by introducing the coloring liquids, it is claimed that it is possible to make the color of frameworks fabricated from zirconium oxide extremely close to the natural tooth color. purpose: the purpose of this research was to evaluate the effect of two staining techniques on value changing in zirconia crowns. materials and method: three groups a, b, and c, each containin...

In order to study of kidney tubules, twenty specimen of 45 days old juvenile grouper (Epinephelus coioides) were prepared after proper biometry. Five mm thick sections were removed from the middle and posterior end of the kidney and fixed in Bouin and glutaraldehyde. The routine procedures of preparation of tissues were followed for light and transmission electron microscopic study. In macrosco...