Purpose: To identify the structures of cellulose-extract derivative (CED) formed by heating Lentinus edodes cellulose in water surroundings that can efficiently inhibit aflatoxin production by Aspergillus flavus. Methods: CED was purified on Sepharose CL-6B columns, and then structurally characterized using amino acid analyzer, gas chromatography, Fourier transform infrared spectroscopy, and po...

Evaluation of Topical Application of Polysaccharide Hemostat and Oxidized Regenerated Cellulose on Epidural Fibrosis in Rats Ahmet Gurhan GURCAY, Atilla KAZANCI, Oktay GURCAN, Ismail BOZKURT, Evrim ONDER, Faruk ALTINEL, Murad BAVBEK Ankara Ataturk Training and Research Hospital, Neurosurgery, Ankara, Turkey Diskapi Yildirim Beyazit Training and Research Hospital, Pathology, Ankara, Turkey Baske...

A plant polysaccharide hydrolase cDNA, designated celD, was isolated from a cDNA library of the rumen fungus Neocallimastix patriciarum. The enzyme encoded by celD had endoglucanase, cellobiohydrolase and xylanase activities. Deletion analysis revealed that celD cDNA can be truncated to code for three catalytically active domains. Each domain had the same substrate specificity as the enzyme pro...

Plant cell wall formation is a complex, coordinated and developmentally regulated process. Cellulose is the most dominant constituent of plant cell walls. Because of its paracrystalline structure, cellulose is the main determinant of mechanical strength of plant tissues. As the most abundant polysaccharide on earth, it is also the focus of cellulosic biofuel industry. To reduce culm lodging in ...

Cellulomonas sp. strain B6 was isolated from a subtropical forest soil sample and presented (hemi)cellulose-degrading activity. We report here its draft genome sequence, with an estimated genome size of 4 Mb, a G+C content of 75.1%, and 3,443 predicted protein-coding sequences, 92 of which are glycosyl hydrolases involved in polysaccharide degradation.

Polysaccharide processing by means of low-temperature Electron Beam Plasma (EBP) is a promising alternative to the time-consuming and environmentally hazardous chemical hydrolysis in oligosaccharide production. The present paper considers mechanisms of the EBP-stimulated destruction of crab shell chitin, cellulose sulfate, and microcrystalline cellulose, as well as characterization of the produ...

Polysaccharide processing by means of low-temperature Electron Beam Plasma (EBP) is a promising alternative to the time-consuming and environmentally hazardous chemical hydrolysis in oligosaccharide production. The present paper considers mechanisms of the EBP-stimulated destruction of crab shell chitin, cellulose sulfate, and microcrystalline cellulose, as well as characterization of the produ...

Intrinsic characteristics of plant cell walls limiting susceptibility of structural carbohydrates to microbial attack in the ruminant's gastrointestinal tract are lignification of the cell wall, covalent bonding of phenolic acids to cell wall polysaccharides, the crystalline structure of cellulose and li mited fibrolytic enzyme accessible space. The exact mechanism by which or degree to which e...

A family 12 endoglucanase with a molecular mass of 23,926 Da (EG-II) from the brown-rot basidiomycete Fomitopsis palustris was purified and characterized. One of the roles of EG-II in wood degradation is thought to be to loosen the polysaccharide network in cell walls by disentangling hemicelluloses that are associated with cellulose.

We demonstrate that two characteristic Sus-like proteins encoded within a polysaccharide utilization locus (PUL) bind strongly to cellulosic substrates and interact with plant primary cell walls. This shows associations between uncultured Bacteroidetes-affiliated lineages and cellulose in the rumen and thus presents new PUL-derived targets to pursue regarding plant biomass degradation.